Enhancing the nutritional quality of major food crops through conventional and genomics-assisted breeding

KB Gaikwad, S Rani, M Kumar, V Gupta… - Frontiers in …, 2020 - frontiersin.org
Nutritional stress is making over two billion world population malnourished. Either our
commercially cultivated varieties of cereals, pulses, and oilseed crops are deficient in …

Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing

WZ Jiang, IM Henry, PG Lynagh… - Plant biotechnology …, 2017 - Wiley Online Library
The CRISPR/Cas9 nuclease system is a powerful and flexible tool for genome editing, and
novel applications of this system are being developed rapidly. Here, we used CRISPR/Cas9 …

CRISPR/Cas9-mediated genome editing of the fatty acid desaturase 2 gene in Brassica napus

A Okuzaki, T Ogawa, C Koizuka, K Kaneko… - Plant Physiology and …, 2018 - Elsevier
The CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9-mediated
genome editing system has been widely applied as a powerful tool for modifying preferable …

CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape (Brassica napus)

J Braatz, HJ Harloff, M Mascher, N Stein… - Plant …, 2017 - academic.oup.com
In polyploid species, altering a trait by random mutagenesis is highly inefficient due to gene
redundancy. We have stably transformed tetraploid oilseed rape (Brassica napus) with a …

The global assessment of oilseed Brassica crop species yield, yield stability and the underlying genetics

JD Zandberg, CT Fernandez, MF Danilevicz… - Plants, 2022 - mdpi.com
The global demand for oilseeds is increasing along with the human population. The family of
Brassicaceae crops are no exception, typically harvested as a valuable source of oil, rich in …

Modifications of fatty acid profile through targeted mutation at BnaFAD2 gene with CRISPR/Cas9-mediated gene editing in Brassica napus

H Huang, T Cui, L Zhang, Q Yang, Y Yang… - Theoretical and Applied …, 2020 - Springer
Key message Genomic editing with CRISPR/Cas9 system can simultaneously modify
multiple copies of the BnaFAD2 gene to develop novel variations in fatty acids profiles in …

Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system

K Zhang, L Nie, Q Cheng, Y Yin, K Chen, F Qi… - Biotechnology for …, 2019 - Springer
Background Brassica napus is one of the most important oilseed crops, and can supply
considerable amounts of edible oil as well as provide raw materials for the production of …

Extensive homoeologous genome exchanges in allopolyploid crops revealed by mRNA seq‐based visualization

Z He, L Wang, AL Harper, L Havlickova… - Plant biotechnology …, 2017 - Wiley Online Library
Polyploidy, the possession of multiple sets of chromosomes, has been a predominant factor
in the evolution and success of the angiosperms. Although artificially formed allopolyploids …

Genome-Wide Association Study of Genetic Control of Seed Fatty Acid Biosynthesis in Brassica napus

K Gacek, PE Bayer, I Bartkowiak-Broda… - Frontiers in Plant …, 2017 - frontiersin.org
Fatty acids and their composition in seeds determine oil value for nutritional or industrial
purposes and also affect seed germination as well as seedling establishment. To better …

Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9-mediated knockout of NtFAD2–2

Y Tian, K Chen, X Li, Y Zheng, F Chen - BMC Plant Biology, 2020 - Springer
Background Tobacco seed oil could be used as an appropriate feedstock for biodiesel
production. However, the high linoleic acid content of tobacco seed oil makes it susceptible …