Volume electron microscopy is the method of choice for the in situ interrogation of cellular
ultrastructure at the nanometer scale, and with the increase in large raw image datasets …

Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and
assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to …

Cryo-soft X-ray tomography (cryo-SXT) is a powerful method to investigate the ultrastructure
of cells, offering resolution in the tens of nanometer range and strong contrast for …

Public participation in research, also known as citizen science, is being increasingly
adopted for the analysis of biological volumetric data. Researchers working in this domain …

Iron is a crucial element integral to various fundamental biological molecular mechanisms,
including magnetosome biogenesis in magnetotactic bacteria (MTB). Magnetosomes are …

Transport in porous media plays an essential role for many physical, engineering, biological
and environmental processes. Novel synchrotron imaging techniques and image-based …

An emergent volume electron microscopy technique called cryogenic serial plasma focused
ion beam milling scanning electron microscopy (pFIB/SEM) can decipher complex biological …

Many bioimaging research projects require objects of interest to be identified, located, and
then traced to allow quantitative measurement. Depending on the complexity of the system …

Summary Segmentation of 3-dimensional (3D, volumetric) images is a widely used
technique that allows interpretation and quantification of experimental data collected using a …

Cochlear hair cell stereocilia bundles are key organelles required for normal hearing. Often,
deafness mutations cause aberrant stereocilia heights or morphology that are visually …