Rational design of enzyme activity and enantioselectivity
The strategy of rational design to engineer enzymes is to predict the potential mutants based
on the understanding of the relationships between protein structure and function, and …
on the understanding of the relationships between protein structure and function, and …
Application of ketoreductase in asymmetric synthesis of pharmaceuticals and bioactive molecules: an update (2018–2020)
Z Li, H Yang, J Liu, Z Huang, F Chen - The Chemical Record, 2021 - Wiley Online Library
With the rapid development of genomic DNA sequencing, recombinant DNA expression,
and protein engineering, biocatalysis has been increasingly and widely adopted in the …
and protein engineering, biocatalysis has been increasingly and widely adopted in the …
Reshaping substrate-binding pocket of leucine dehydrogenase for bidirectionally accessing structurally diverse substrates
T Wu, Y Wang, N Zhang, D Yin, Y Xu, Y Nie, X Mu - ACS Catalysis, 2022 - ACS Publications
Steric hindrance modification-based pocket reshaping is an effective approach for enzyme–
substrate acceptance evolution. However, previous studies were limited to using the …
substrate acceptance evolution. However, previous studies were limited to using the …
Microbial alcohol dehydrogenases: recent developments and applications in asymmetric synthesis
Alcohol dehydrogenases are a well-known group of enzymes in the class of
oxidoreductases that use electron transfer cofactors such as NAD (P)+/NAD (P) H for …
oxidoreductases that use electron transfer cofactors such as NAD (P)+/NAD (P) H for …
Stereochemistry in asymmetric reduction of bulky–bulky ketones by alcohol dehydrogenases
J Zhou, G Xu, Y Ni - ACS Catalysis, 2020 - ACS Publications
Biocatalyzed asymmetric reduction of ketones is one of the most cost-effective and
environmentally friendly approaches for preparing chiral alcohols. However, few natural …
environmentally friendly approaches for preparing chiral alcohols. However, few natural …
Batch and continuous flow asymmetric synthesis of anabolic-androgenic steroids via a single-cell biocatalytic Δ 1-dehydrogenation and C17β-carbonyl reduction …
Y Zhang, M Liu, Z Yang, J Lin, Z Huang, F Chen - Green Chemistry, 2023 - pubs.rsc.org
Chemoenzymatic asymmetric synthesis of an anabolic-androgenic steroid (+)-boldenone (3)
and its prodrug (+)-boldenone undecylenate (4) was accomplished starting from …
and its prodrug (+)-boldenone undecylenate (4) was accomplished starting from …
Computer-aided understanding and engineering of enzymatic selectivity
L Wu, L Qin, Y Nie, Y Xu, YL Zhao - Biotechnology Advances, 2022 - Elsevier
Abstract Enzymes offering chemo-, regio-, and stereoselectivity enable the asymmetric
synthesis of high-value chiral molecules. Unfortunately, the drawback that naturally …
synthesis of high-value chiral molecules. Unfortunately, the drawback that naturally …
From semirational to rational design: developing a substrate-coupled system of glucose dehydrogenase for asymmetric synthesis
Q Yan, X Zhang, Y Chen, B Guo, P Zhou, B Chen… - ACS …, 2022 - ACS Publications
Glucose dehydrogenase (GDH) generally functions as an expensive cofactor NAD (P) H
regenerator in an enzyme-coupled cofactor regeneration system, as in the production of fine …
regenerator in an enzyme-coupled cofactor regeneration system, as in the production of fine …
Engineered Glycosidase for Significantly Improved Production of Naturally Rare Vina-Ginsenoside R7
Ginsenosides are the main bioactive ingredients in plants of the genus Panax. Vina-
ginsenoside R7 (VG-R7) is one of the rare high-value ginsenosides with health benefits. The …
ginsenoside R7 (VG-R7) is one of the rare high-value ginsenosides with health benefits. The …
An enzyme-assembled gel monolithic microreactor for continuous flow asymmetric synthesis of aryl alcohols
Green, efficient, and sustainable flow synthesis of intermediate chiral aryl alcohols is critical
for continuous drug-manufacturing. Enzyme immobilization endows flow biocatalysis with …
for continuous drug-manufacturing. Enzyme immobilization endows flow biocatalysis with …