Drought is a crucial environmental constraint limiting crop production in many parts of the
world. microRNA (miRNA) based gene regulation has been shown to act in several …

Background An important step in characterising the function of a gene is identifying the cells
in which it is expressed. Traditional methods to determine this include in situ hybridisation …

The genetic basis of the regeneration process in cultured immature embryos of rye (Secale
cereale L.) was analyzed. The experiments were designed to reveal differences between the …

Background Ericoid mycorrhizal fungi (EMF) play important roles in mineral cycling and
plant nutrient acquisition, and they increase plant survival in nutrient-poor environments. In …

K+ uptake to root conducting cells is a prerequisite for its release to the xylem vessels and
long-distance delivery to the aerial parts, though the molecular mechanism underpinning …

In situ PCR is a technique that allows specific nucleic acid sequences to be detected in
individual cells and tissues. In situ PCR and IS‐RT‐PCR are elegant techniques that can …

Abstrakt AUXIN RESPONSE FACTOR (ARF) proteiny představují velkou rodinu
transkripčních faktorů, jejichž funkcí je aktivace nebo represe genů primární odpovědi na …

Figure S4. The sequencing verification of miRNA stem-loop qRT-PCR products (ak).
Reverse complimentary sequence of the forward primers for respective miRNA is shown in …

Increasing soil salinity of agricultural land is of growing concern world-wide as excessive
soil salinity has a detrimental effect on growth and yield of many plant species of agricultural …

In situ PCR is a technique, which allows detection of specific nucleic acid sequences in
particular cells and tissues. In plants, in situ RT-PCR is widely used in localisation of specific …