Single-molecule, real-time sequencing developed by Pacific BioSciences offers longer read
lengths than the second-generation sequencing (SGS) technologies, making it well-suited …

Structural variants (SVs) can contribute to oncogenesis through a variety of mechanisms.
Despite their importance, the identification of SVs in cancer genomes remains challenging …

Historically, human breast cancer has been modeled largely in vitro using long-established
cell lines primarily in two-dimensional culture, but also in three-dimensional cultures of …

We developed an innovative hybrid sequencing approach, IDP-fusion, to detect fusion
genes, determine fusion sites and identify and quantify fusion isoforms. IDP-fusion is the first …

Background It has recently emerged that common epithelial cancers such as breast cancers
have fusion genes like those in leukaemias. In a representative breast cancer cell line, ZR …

Eliminating the bacterial cloning step has been a major factor in the vastly improved
efficiency of massively parallel sequencing approaches. However, this also has made it a …

Overexpression of human epidermal growth factor receptor-2 (HER-2) occurs in 20% of all
breast cancer subtypes, especially those that present the worst prognostic outcome through …

Background Paired-tag sequencing approaches are commonly used for the analysis of
genome structure. However, mammalian genomes have a complex organization with a …

We present the discovery of genes recurrently involved in structural variation in
nasopharyngeal carcinoma (NPC) and the identification of a novel type of somatic structural …

For many years, it was assumed that gene fusions were a type of mutation confined largely
to leukemias and sarcomas. However, fusion genes are now known to be important in …