Although dynamic self-assembly, DySA, is a relatively new area of research, the past
decade has brought numerous demonstrations of how various types of components–on …

Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

The physical nature of the bacterial cytoplasm is poorly understood even though it
determines cytoplasmic dynamics and hence cellular physiology and behavior. Through …

In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria
has vaulted from obscurity to become one of the most widely studied and exploited proteins …

Optogenetics allows manipulations of genetically and spatially defined neuronal populations
with excellent temporal control. However, neurons are coupled with other neurons over …

Biological structures span many orders of magnitude in size, but far-field visible light
microscopy suffers from limited resolution. A new method for fluorescence imaging has been …

Little is known about how neutrophils and other cells establish a single zone of actin
assembly during migration. A widespread assumption is that the leading edge prevents …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Classical biochemistry is founded on several assumptions valid in dilute aqueous solutions
that are often extended without question to the interior milieu of intact cells. In the first section …

A longstanding question in molecular biology is the extent to which the behavior of
macromolecules observed in vitro accurately reflects their behavior in vivo. A number of …