Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …

Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …

Therapeutic applications of nuclease-based genome editing would benefit from improved
methods for transgene integration via homology-directed repair (HDR). To improve HDR …

Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-
associated (Cas) system has emerged as the main technology for gene editing. Successful …

In the aquaculture industry, selective breeding has played an important role in increasing
aquaculture production significantly. To meet the global growing demand for high quality …

The first step in CRISPR-Cas9-mediated genome editing is the cleavage of target DNA
sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs …

GM2 gangliosidoses are a group of pathologies characterized by GM2 ganglioside
accumulation into the lysosome due to mutations on the genes encoding for the β …

Since its inception, the CRISPR/Cas9 technology has been widely utilized for the targeted
insertion of donor DNAs into mammalian genomes. A shortcoming with the earlier knock-in …

Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic
engineering tool in various biological systems. This editing technology has been in the …

Introduction of CRISPR/Cas9 methods (clustered regularly interspaced short palindromic
repeats, CRISPR-associated protein 9) have led to a huge surge in the use of precision …