Highlights•Growing application of continuous processing in manufacturing protein
therapeutics.•Developing continuous processing for viral vector, vaccine, and cell therapy …

Surface plasmon resonance (SPR)-based optical biosensors offer real-time and label-free
analysis of protein interactions, which has extensively contributed to the discovery and …

Raman spectrum based predictive models provide a process analytical technology (PAT)
tool for monitoring and control of culture parameters in bioprocesses. Steady-state perfusion …

In this study, we demonstrated the first, to our knowledge, integrated continuous bioprocess
(ICB) designed for the production of acid‐sensitive monoclonal antibodies, prone to …

A novel approach of design of experiment (DoE) is developed for the optimization of key
substrates of the culture medium, amino acids, and sugars, by utilizing perfusion …

Antibody based drugs, including IgG monoclonal antibodies, are an expanding class of
therapeutics widely employed to treat cancer, autoimmune and infectious diseases. IgG …

Background Changes in IgG glycosylation, as a novel pathological feature, are observed in
various autoimmune diseases (AIDs). The glycosylation patterns of IgG play a critical role in …

Highlights•Temporal evolution of N-glycosylation is of rising interest.•Rapid detection with
predictive modeling could enable dynamic control of N-glycosylation.•Combinations of …

BACKGROUND Tangential flow filtration (TFF) systems are widely used cell retention
devices in perfusion cultures, but significant challenges occur with their prolonged …

The perfusion medium is critical in maintaining high cell concentration in cultures for the
production of monoclonal antibody by Chinese hamster ovary cells. In this study, the effects …