Redox Regulation and Oxidative Stress in Mammalian Oocytes and Embryos Developed In Vivo and In Vitro

MLM Hardy, ML Day, MB Morris - International Journal of Environmental …, 2021 - mdpi.com
Oocytes and preimplantation embryos require careful regulation of the redox environment
for optimal development both in vivo and in vitro. Reactive oxygen species (ROS) are …

Cryopreservation of mammalian embryos and oocytes: recent advances

AT Palasz, RJ Mapletoft - Biotechnology Advances, 1996 - Elsevier
The cryopreservation of embryos of most domestic species has become a routine procedure
in embryo transfer, and recently, advances have been made in the cold storage of …

Superovulatory response following ablation-induced follicular wave emergence at random stages of the oestrous cycle in cattle

DR Bergfelt, GA Bo, RJ Mapletoft, GP Adams - Animal Reproduction …, 1997 - Elsevier
Based on the premise that superovulation in cattle is optimal when superstimulation is
initiated at the time of follicular wave emergence, the present study was done in beef heifers …

Handling individual mammalian embryos using microfluidics

IK Glasgow, HC Zeringue, DJ Beebe… - IEEE transactions on …, 2001 - ieeexplore.ieee.org
We have designed, built, and tested microfluidic systems capable of transporting individual,
preimplantation mouse embryos (100-/spl mu/mm to 150-/spl mu/m diameter) through a …

Polyvinyl alcohol as a defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development

S Naitana, S Ledda, P Loi, G Leoni, L Bogliolo… - Animal Reproduction …, 1997 - Elsevier
The purpose of this study was to assess the viability of ovine embryos after replacing fetal
calf serum (FCS) with polyvinyl alcohol (PVA) in vitrification and warming solutions. Ovine …

[PDF][PDF] In vivo survival rate of rabbit morulae after vitrification in a medium without serum protein

JS Vicente, MP Viudes-De-Castro… - Reproduction Nutrition …, 1999 - rnd.edpsciences.org
The in vivo survival rate of rabbit morulae after vitrification in a mixture of dimethyl
sulphoxide and ethylene glycol solution without protein supplement (WPS) was compared …

Sanitary risks related to embryo transfer in domestic species

B Guerin, M Nibart, B Marquant-Le Guienne, P Humblot - Theriogenology, 1997 - Elsevier
When using embryo transfer (ET), sperm and oocytes are potential sources of pathogens
(bacteria, viruses, prions). Additionally, contamination may result from environmental …

The effect of macromolecular supplementation on the surface tension of TCM-199 and the utilization of growth factors by bovine oocytes and embryos in culture

AT Palasz, J Thundathil, RE Verrall… - Animal reproduction …, 2000 - Elsevier
The objectives of this study were to determine the surface tension of bovine follicular fluid
(BFF) and TCM-199, and the effects of the synthetic surfactant, Twin-80, or FCS, in TCM-199 …

The protective action of polyvinyl alcohol during rapid-freezing of mouse embryos

MA Nowshari, G Brem - Theriogenology, 2000 - Elsevier
Biological products like serum and BSA are routinely used in embryo freezing solutions.
These products are undefined and can potentially expose the embryos to infectious agents …

Improved cryopreservation of in vitro-produced bovine embryos using a chemically defined freezing medium

P Bruyère, A Baudot, C Guyader-Joly, P Guérin… - Theriogenology, 2012 - Elsevier
This study evaluates a new synthetic substitute (CRYO3, Ref. 5617, Stem Alpha, France) for
animal-based products in bovine embryo cryopreservation solutions. During the experiment …