Novel strategies to address the challenges in top-down proteomics

JA Melby, DS Roberts, EJ Larson… - Journal of the …, 2021 - ACS Publications
Top-down mass spectrometry (MS)-based proteomics is a powerful technology for
comprehensively characterizing proteoforms to decipher post-translational modifications …

Protein purification and analysis: next generation Western blotting techniques

M Mishra, S Tiwari, AV Gomes - Expert review of proteomics, 2017 - Taylor & Francis
Introduction: Western blotting is one of the most commonly used techniques in molecular
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites

V Akimov, I Barrio-Hernandez, SVF Hansen… - Nature structural & …, 2018 - nature.com
Ubiquitination is a post-translational modification (PTM) that is essential for balancing
numerous physiological processes. To enable delineation of protein ubiquitination at a site …

Serological cross-reactivity among common flaviviruses

KR Chan, AA Ismail, G Thergarajan, CS Raju… - Frontiers in Cellular …, 2022 - frontiersin.org
The Flavivirus genus is made up of viruses that are either mosquito-borne or tick-borne and
other viruses transmitted by unknown vectors. Flaviviruses present a significant threat to …

Tubulin or not tubulin: heading toward total protein staining as loading control in western blots

CP Moritz - Proteomics, 2017 - Wiley Online Library
Western blotting is an analytical method widely used for detecting and (semi‐) quantifying
specific proteins in given samples. Western blots are continuously applied and developed …

[HTML][HTML] Proteomes are of proteoforms: embracing the complexity

K Carbonara, M Andonovski, JR Coorssen - Proteomes, 2021 - mdpi.com
Proteomes are complex—much more so than genomes or transcriptomes. Thus, simplifying
their analysis does not simplify the issue. Proteomes are of proteoforms, not canonical …

[HTML][HTML] Role of ingested amino acids and protein in the promotion of resistance exercise–induced muscle protein anabolism

PT Reidy, BB Rasmussen - The Journal of nutrition, 2016 - Elsevier
The goal of this critical review is to comprehensively assess the evidence for the molecular,
physiologic, and phenotypic skeletal muscle responses to resistance exercise (RE) …

Ponceau S waste: Ponceau S staining for total protein normalization

H Sander, S Wallace, R Plouse, S Tiwari… - Analytical biochemistry, 2019 - Elsevier
Normalization of Western blotting data is a critical step that is needed to reduce errors
caused by unequal sample loading across lanes in a gel, inconsistent sample preparation …

Western blotting: a powerful staple in scientific and biomedical research

H Begum, P Murugesan, AD Tangutur - Biotechniques, 2022 - Taylor & Francis
Western blotting (WB), also known as immunoblotting, is a well-known molecular biology
method that biologists often use to investigate many features of the protein, ranging from …

When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions

ARM Bradbury, ND Trinklein, H Thie, IC Wilkinson… - MAbs, 2018 - Taylor & Francis
Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies
have reported genetic diversity in antibody heavy chain and light chain genes found within …