Enzymatic hydrolysis is a critical process for the cellulase-mediated lignocellulosic
biorefinery to produce sugar syrups that can be converted into a whole range of biofuels and …

Highlights•New enzymes have revolutionized the liquefaction process in starch
ethanol.•New enzyme products have improved ethanol yield and product …

Bioconversion of lignocellulose forms the basis for renewable, advanced biofuels, and
bioproducts. Mechanisms of hydrolysis of cellulose by cellulases have been actively studied …

Highlights•Understanding of the mechanisms of the enzymes involved has been
substantially improved.•Successful shaping of the enzymes for the industrial process has …

This study aimed to efficiently convert banana peels (BP) into 5-hydroxymethylfurfural (HMF)
by using an integrated mechanoenzymatic/catalytic approach. There is no report on HMF …

The traditional way of rationally engineering enzymes to change their biocatalytic properties
utilizes the modifications of their active sites. Another emerging approach is the engineering …

Cellobiohydrolases are exoacting, processive enzymes that effectively hydrolyze crystalline
cellulose. They have attracted considerable interest because of their role in both natural …

Cellobiohydrolases are exo-active glycosyl hydrolases that processively convert cellulose to
soluble sugars, typically cellobiose. They effectively break down crystalline cellulose and …

Interfacial enzyme reactions require formation of an enzyme–substrate complex at the
surface of a heterogeneous substrate, but often multiple modes of enzyme binding and types …

Processive hydrolysis of crystalline cellulose by cellulases is a critical step for lignocellulose
deconstruction. The classic Trichoderma reesei exoglucanase Tr Cel7A, which has a closed …