Antibiotic resistance in wastewater treatment plants: Tackling the black box

CM Manaia, J Rocha, N Scaccia, R Marano… - Environment …, 2018 - Elsevier
Wastewater is among the most important reservoirs of antibiotic resistance in urban
environments. The abundance of carbon sources and other nutrients, a variety of possible …

[HTML][HTML] Monitoring antibiotic resistance genes in wastewater environments: the challenges of filling a gap in the One-Health cycle

A Miłobedzka, C Ferreira, I Vaz-Moreira… - Journal of Hazardous …, 2022 - Elsevier
Antibiotic resistance (AR) is a global problem requiring international cooperation and
coordinated action. Global monitoring must rely on methods available and comparable …

Long-read metagenomic sequencing reveals shifts in associations of antibiotic resistance genes with mobile genetic elements from sewage to activated sludge

D Dai, C Brown, H Bürgmann, DGJ Larsson, I Nambi… - Microbiome, 2022 - Springer
Background There is concern that the microbially rich activated sludge environment of
wastewater treatment plants (WWTPs) may contribute to the dissemination of antibiotic …

Using sewage for surveillance of antimicrobial resistance

FM Aarestrup, MEJ Woolhouse - Science, 2020 - science.org
Antimicrobial resistance (AMR), a cross-cutting and increasing threat to global health (1–3),
is a complex problem with multiple and interconnected drivers. Reliable surveillance data …

A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater

I Keenum, K Liguori, J Calarco, BC Davis… - Critical Reviews in …, 2022 - Taylor & Francis
Water environments are increasingly recognized as a conduit for the spread of antibiotic
resistance, but there is need to standardize antibiotic resistance monitoring protocols to …

Whole genome sequencing and metagenomics for outbreak investigation, source attribution and risk assessment of food‐borne microorganisms

EFSA Panel on Biological Hazards (EFSA … - EFSA …, 2019 - Wiley Online Library
This Opinion considers the application of whole genome sequencing (WGS) and
metagenomics for outbreak investigation, source attribution and risk assessment of food …

The present and future of whole genome sequencing (WGS) and whole metagenome sequencing (WMS) for surveillance of antimicrobial resistant microorganisms …

EA Oniciuc, E Likotrafiti, A Alvarez-Molina, M Prieto… - Genes, 2018 - mdpi.com
Antimicrobial resistance (AMR) surveillance is a critical step within risk assessment
schemes, as it is the basis for informing global strategies, monitoring the effectiveness of …

Critical evaluation of short, long, and hybrid assembly for contextual analysis of antibiotic resistance genes in complex environmental metagenomes

CL Brown, IM Keenum, D Dai, L Zhang, PJ Vikesland… - Scientific reports, 2021 - nature.com
In the fight to limit the global spread of antibiotic resistance, the assembly of environmental
metagenomes has the potential to provide rich contextual information (eg, taxonomic hosts …

Molecular methods for pathogenic bacteria detection and recent advances in wastewater analysis

S Zhang, X Li, J Wu, L Coin, J O'brien, F Hai, G Jiang - Water, 2021 - mdpi.com
With increasing concerns about public health and the development of molecular techniques,
new detection tools and the combination of existing approaches have increased the abilities …

Recommendations for the use of metagenomics for routine monitoring of antibiotic resistance in wastewater and impacted aquatic environments

BC Davis, C Brown, S Gupta, J Calarco… - Critical Reviews in …, 2023 - Taylor & Francis
Shotgun metagenomic sequencing of the collective genomic information carried across
microbial communities is emerging as a powerful approach for monitoring antibiotic …