Natural bacterial transformation involves the internalization and chromosomal integration of
DNA and has now been documented in∼ 80 species. Recent advances have established …

It is expected that the increasing genetic information on Bacillus cereus in genome
databases will pave the way for investigating its evolution, ecology, and virulence, and …

Despite the development of several cultivation methods, the rate of discovery of
microorganisms that are yet-to-be cultivated outpaces the rate of isolating and cultivating …

Bacillus subtilis can serve as a powerful platform for directed evolution, especially for
secretory enzymes. However, cloning and transformation of a DNA mutant library in B …

The genus Geobacillus comprises a group of Gram-positive thermophilic bacteria, including
obligate aerobes, denitrifiers, and facultative anaerobes that can grow over a range of 45 …

It has long been a question whether Staphylococcus aureus, a major human pathogen, is
able to develop natural competence for transformation by DNA. We previously showed that a …

The Bacillus cereus group, also known as B. cereus sensu lato, is a subdivision of the
Bacillus genus that consists of eight formally recognised species: B. cereus sensu stricto, B …

To mimic the Escherichia coli T7 protein expression system, we developed a facile T7
promoter-based protein expression system in an industrial microorganism Bacillus subtilis …

Lactic acid (LA) is a significant byproduct in Baijiu fermentation, with both isomers of LA and
ethyl lactate (EL) being present in strong-aroma Baijiu. D (−)-isomers cannot be metabolized …

Streptococcussuis is an important zoonotic pathogen that causes severe invasive disease in
pigs and humans. Current methods for genome engineering of S. suis rely on the insertion of …