The solvent side of proteinaceous membrane-less organelles in light of aqueous two-phase systems

BY Zaslavsky, LA Ferreira, AL Darling… - International journal of …, 2018 - Elsevier
Water represents a common denominator for liquid-liquid phase transitions leading to the
formation of the polymer-based aqueous two-phase systems (ATPSs) and a set of the …

Analytical applications of partitioning in aqueous two-phase systems: Exploring protein structural changes and protein–partner interactions in vitro and in vivo by …

BY Zaslavsky, VN Uversky, A Chait - … et Biophysica Acta (BBA)-Proteins and …, 2016 - Elsevier
This review covers the fundamentals of protein partitioning in aqueous two-phase systems
(ATPS). Included is a review of advancements in the analytical application of solute …

Salting-in and salting-out of water-soluble polymers in aqueous salt solutions

R Sadeghi, F Jahani - The Journal of Physical Chemistry B, 2012 - ACS Publications
To obtain further experimental evidence for the mechanisms of the salting effect produced by
the addition of salting-out or sating-in inducing electrolytes to aqueous solutions of water …

Aqueous two phase extraction for purification of C-phycocyanin

G Patil, K Raghavarao - Biochemical Engineering Journal, 2007 - Elsevier
Aqueous two phase extraction is employed for the purification of C-phycocyanin from
Spirulina platensis. A systematic approach is suggested to arrive at the optimal process …

Programmable hydrogel ionic circuits for biologically matched electronic interfaces

S Zhao, P Tseng, J Grasman, Y Wang, W Li… - Advanced …, 2018 - Wiley Online Library
The increased need for wearable and implantable medical devices has driven the demand
for electronics that interface with living systems. Current bioelectronic systems have not fully …

Extraction and purification of lipase from Enterococcus faecium MTCC5695 by PEG/phosphate aqueous-two phase system (ATPS) and its biochemical …

V Ramakrishnan, LC Goveas, N Suralikerimath… - Biocatalysis and …, 2016 - Elsevier
Intracellular lipase from Enterococcus faecium MTCC5695 (MTCC5695), a lactic acid
bacterium, grown at optimal conditions was subjected to purification and concentration using …

Extractive fermentation for the production and partitioning of lipase and citric acid by Yarrowia lipolytica

AG Santos, FS Buarque, BD Ribeiro, MAZ Coelho - Process Biochemistry, 2022 - Elsevier
Extractive fermentation is an efficient downstream process to segregate the desired product
in a fermentative process. Here, an integrated extractive fermentation process with an …

Liquid–liquid equilibrium of aqueous two-phase system composed of poly (ethylene oxide) 1500 and sodium nitrate

YA Zakhodyaeva, DG Rudakov… - Journal of Chemical & …, 2019 - ACS Publications
Liquid–liquid equilibria of an aqueous two-phase system consisting of poly (ethylene oxide)
1500, sodium nitrate, and water at 293.15, 313.15, and 333.15 K were studied. Binodal data …

A multisolute osmotic virial equation for solutions of interest in biology

JAW Elliott, RC Prickett, HY Elmoazzen… - The Journal of …, 2007 - ACS Publications
The osmotic virial equation was used to predict osmolalities of solutions of interest in
biology. The second osmotic virial coefficients, B i, account for the interactions between …

Bovine serum albumin partitioning in polyethylene glycol (PEG)/potassium citrate aqueous two-phase systems

YM Lu, YZ Yang, XD Zhao, CB Xia - Food and bioproducts processing, 2010 - Elsevier
The extraction and back-extraction of bovine serum albumin (BSA) have been studied by
liquid–liquid extraction with poly (ethylene glycol)(PEG)/potassium citrate aqueous two …