After three decades of developments, single particle tracking (SPT) has become a powerful
tool to interrogate dynamics in a range of materials including live cells and novel catalytic …

Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

Individual proteins and protein complexes undergo various motion types, including free
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …

We examine the capability of mean square displacement (MSD) analysis to extract reliable
values of the diffusion coefficient D of a single particle undergoing Brownian motion in an …

Molecular transport in living systems regulates numerous processes underlying biological
function. Although many cellular components exhibit anomalous diffusion, only recently has …

Single-particle tracking is increasingly used to extract quantitative parameters on single
molecules and their environment, while advances in spatial and temporal resolution of …

Native mucus is polymer-based soft-matter material of paramount biological importance.
How non-Gaussian and non-ergodic is the diffusive spreading of pathogens in mucus? We …

Highly sensitive fluorescence microscopy techniques allow single nanoparticles to be
tracked during their uptake into living cells with high temporal and spatial resolution. From …

Camera-based single-particle tracking enables quantitative determination of transport
properties and provides nanoscale information about material characteristics such as …

A detailed understanding of the cellular uptake process is essential to the development of
cellular delivery strategies and to the study of viral trafficking. However, visualization of the …