Traditionally, structural biologists approach the complexity of cellular proteomes in a
reductionist manner. Proteomes are fractionated, their molecular components purified and …

Biological function of macromolecules is closely tied to their cellular location, as well as to
interactions with other molecules within the native environment of the cell. Therefore, to …

Cryo-electron tomography allows the routine visualization of cellular landscapes in three
dimensions at nanometer-range resolutions. When combined with single-particle …

Cryo-electron tomography (CryoET) images the cellular environment in situ, without labels,
and with fully preserved context. However, the complex cellular environment and the low …

The combination of cryo-electron tomography and subtomogram analysis affords 3D high-
resolution views of biological macromolecules in their native cellular environment, or in situ …

Determining the structure and mechanisms of all individual functional modules of cells at
high molecular detail has often been seen as equal to understanding how cells work …

Cryo-electron tomography can uniquely probe the native cellular environment for
macromolecular structures. Tomograms feature complex data with densities of diverse …

Understanding the in situ structure, organization, and interactions of macromolecules is
essential for elucidating their functions and mechanisms of action. Cellular cryo-electron …

The three-dimensional organization of biomolecules important for the functioning of all living
systems can be determined by cryo-electron tomography imaging under native biological …

In many real-life image analysis applications, particularly in biomedical research domains,
the objects of interest undergo multiple transformations that alters their visual properties …