Human organoids in basic research and clinical applications

XY Tang, S Wu, D Wang, C Chu, Y Hong… - Signal transduction and …, 2022 - nature.com
Organoids are three-dimensional (3D) miniature structures cultured in vitro produced from
either human pluripotent stem cells (hPSCs) or adult stem cells (AdSCs) derived from …

The promises and challenges of human brain organoids as models of neuropsychiatric disease

G Quadrato, J Brown, P Arlotta - Nature medicine, 2016 - nature.com
Neuropsychiatric disorders such as autism spectrum disorder (ASD), schizophrenia (SCZ)
and bipolar disorder (BPD) are of great societal and medical importance, but the complexity …

Modeling Parkinson's disease in midbrain-like organoids

LM Smits, L Reinhardt, P Reinhardt, M Glatza… - NPJ Parkinson's …, 2019 - nature.com
Modeling Parkinson's disease (PD) using advanced experimental in vitro models is a
powerful tool to study disease mechanisms and to elucidate unexplored aspects of this …

Millifluidic culture improves human midbrain organoid vitality and differentiation

E Berger, C Magliaro, N Paczia, AS Monzel, P Antony… - Lab on a Chip, 2018 - pubs.rsc.org
Human midbrain-specific organoids (hMOs) serve as an experimental in vitro model for
studying the pathogenesis of Parkinson's disease (PD). In hMOs, neuroepithelial stem cells …

Stem cell models of Alzheimer's disease: progress and challenges

C Arber, C Lovejoy, S Wray - Alzheimer's research & therapy, 2017 - Springer
A major challenge to our understanding of the molecular mechanisms of Alzheimer's
disease (AD) has been the lack of physiologically relevant in vitro models which capture the …

[HTML][HTML] Studying human neurological disorders using induced pluripotent stem cells: from 2D monolayer to 3D organoid and blood brain barrier models

S Logan, T Arzua, SG Canfield… - Comprehensive …, 2019 - ncbi.nlm.nih.gov
Neurological disorders have emerged as a predominant healthcare concern in recent years
due to their severe consequences on quality of life and prevalence throughout the world …

In vitro acute and developmental neurotoxicity screening: an overview of cellular platforms and high-throughput technical possibilities

BZ Schmidt, M Lehmann, S Gutbier, E Nembo… - Archives of …, 2017 - Springer
Neurotoxicity and developmental neurotoxicity are important issues of chemical hazard
assessment. Since the interpretation of animal data and their extrapolation to man is …

Modelling human brain development and disease with organoids

M Birtele, M Lancaster, G Quadrato - Nature Reviews Molecular Cell …, 2024 - nature.com
Organoids are systems derived from pluripotent stem cells at the interface between
traditional monolayer cultures and in vivo animal models. The structural and functional …

Generation of homogeneous midbrain organoids with in vivo-like cellular composition facilitates neurotoxin-based Parkinson's disease modeling

TH Kwak, JH Kang, S Hali, J Kim, KP Kim, C Park… - Stem …, 2020 - academic.oup.com
Recent studies have demonstrated the generation of midbrain-like organoids (MOs) from
human pluripotent stem cells. However, the low efficiency of MO generation and the …

[HTML][HTML] A matrigel-free method to generate matured human cerebral organoids using 3D-Printed microwell arrays

C Chen, V Rengarajan, A Kjar, Y Huang - Bioactive materials, 2021 - Elsevier
The current methods of generating human cerebral organoids rely excessively on the use of
Matrigel or other external extracellular matrices (ECM) for cell micro-environmental …