Next-generation sequencing (NGS) technology has expanded in the last decades with
significant improvements in the reliability, sequencing chemistry, pipeline analyses, data …

A comprehensive understanding of host–pathogen interactions requires a knowledge of the
associated gene expression changes in both the pathogen and the host. Traditional, probe …

High-throughput complementary DNA sequencing technologies have advanced our
understanding of transcriptome complexity and regulation. However, these methods lose …

Background RNA-Seq is revolutionizing the way transcript abundances are measured. A key
challenge in transcript quantification from RNA-Seq data is the handling of reads that map to …

Neuroblastoma is a malignancy of the developing sympathetic nervous system that often
presents with widespread metastatic disease, resulting in survival rates of less than 50%. To …

Cellular processes often depend on stable physical associations between proteins. Despite
recent progress, knowledge of the composition of human protein complexes remains limited …

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-
sequencing technologies. Studies using this method have already altered our view of the …

Although it is known that the methylation of DNA in 5′ promoters suppresses gene
expression, the role of DNA methylation in gene bodies is unclear,,,,. In mammals, tissue …

The human mitochondrial genome comprises a distinct genetic system transcribed as
precursor polycistronic transcripts that are subsequently cleaved to generate individual …