Protein phosphorylation is a post-translational modification (PTM) that orchestrates a diverse
array of cellular processes. Because this modification serves as a rapid and reversible …

Many pressing medical challenges, such as diagnosing disease, enhancing directed stem-
cell differentiation, and classifying cancers, have long been hindered by limitations in our …

Pancreatic and lung cancers frequently develop resistance to chemotherapy-induced cell
apoptosis during the treatment, indicating that targeting nonapoptotic-related pathways …

Bottom-up proteomic strategies rely on efficient digestion of proteins into peptides for mass
spectrometry analysis. In-solution and filter-based strategies are commonly used for …

We have reported a set of electrokinetically pumped sheath flow nanoelectrospray interfaces
to couple capillary zone electrophoresis with mass spectrometry. A separation capillary is …

Mass spectrometry (MS) has become an increasingly important technique to analyze
proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine …

Multilevel proteomics aims to delineate proteins at the peptide (bottom‐up proteomics),
proteoform (top‐down proteomics), and protein complex (native proteomics) levels. Capillary …

In this work, we developed an ultra-sensitive CE-MS/MS method for bottom-up proteomics
analysis of limited samples, down to sub-nanogram levels of total protein. Analysis of 880 …

Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-
MS/MS) has been recognized as an invaluable platform for top-down proteomics. However …

This review focuses on the latest development of microseparation electromigration methods
in capillaries and microfluidic devices coupled with MS for detection and identification of …