Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Fluorescence lifetime imaging microscopy
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …
insights into a broad spectrum of biological processes such as metabolic imaging, protein …
Wide-field fluorescence lifetime imaging of single molecules
N Oleksiievets, JC Thiele, A Weber… - The Journal of …, 2020 - ACS Publications
Fluorescence lifetime imaging (FLIM) has become an important microscopy technique in
bioimaging. The two most important of its applications are lifetime-multiplexing for imaging …
bioimaging. The two most important of its applications are lifetime-multiplexing for imaging …
Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems
From individual cells to whole organisms, O 2 transport unfolds across micrometer-to
millimeter-length scales and can change within milliseconds in response to fluid flows and …
millimeter-length scales and can change within milliseconds in response to fluid flows and …
Förster resonance energy transfer–what can we learn and how can we use it?
H Schneckenburger - Methods and applications in fluorescence, 2019 - iopscience.iop.org
The present manuscript gives a short overview on Förster Resonance Energy Transfer
(FRET) of molecular interactions in the nanometre range. First, its principle is described and …
(FRET) of molecular interactions in the nanometre range. First, its principle is described and …
High-throughput, multi-parametric, and correlative fluorescence lifetime imaging
In this review, we discuss methods and advancements in fluorescence lifetime imaging
microscopy that permit measurements to be performed at faster speed and higher resolution …
microscopy that permit measurements to be performed at faster speed and higher resolution …
Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
AJ Bowman, BB Klopfer, T Juffmann… - Nature …, 2019 - nature.com
Nanosecond temporal resolution enables new methods for wide-field imaging like time-of-
flight, gated detection, and fluorescence lifetime. The optical efficiency of existing …
flight, gated detection, and fluorescence lifetime. The optical efficiency of existing …
Luminescence lifetime imaging of chemical sensors—A comparison between time-domain and frequency-domain based camera systems
Luminescence lifetime based imaging is still the most reliable method for generating
chemical images using chemical sensor technology. However, only few commercial systems …
chemical images using chemical sensor technology. However, only few commercial systems …
Label-free fluorescence microscopy: revisiting the opportunities with autofluorescent molecules and harmonic generations as biosensors and biomarkers for …
Over the past decade, the utilization of advanced fluorescence microscopy technologies has
presented numerous opportunities to study or re-investigate autofluorescent molecules and …
presented numerous opportunities to study or re-investigate autofluorescent molecules and …
Nanoparticle-and microparticle-based luminescence imaging of chemical species and temperature in aquatic systems: a review
Most aquatic systems rely on a multitude of biogeochemical processes that are coupled with
each other in a complex and dynamic manner. To understand such processes, minimally …
each other in a complex and dynamic manner. To understand such processes, minimally …