Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Recent advances in stimuli‐responsive shape‐morphing hydrogels

X Liu, M Gao, J Chen, S Guo, W Zhu… - Advanced Functional …, 2022 - Wiley Online Library
Inspired by shape‐morphing organisms in nature, researchers have developed various
hydrogels with stimuli‐responsive swelling, shrinking, bending, folding, origami, rolling …

Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy

W Zhao, S Zhao, L Li, X Huang, S Xing, Y Zhang… - Nature …, 2022 - nature.com
A main determinant of the spatial resolution of live-cell super-resolution (SR) microscopes is
the maximum photon flux that can be collected. To further increase the effective resolution …

[HTML][HTML] 3D printing of hydrogels: Rational design strategies and emerging biomedical applications

J Li, C Wu, PK Chu, M Gelinsky - Materials Science and Engineering: R …, 2020 - Elsevier
Abstract 3D printing alias additive manufacturing can transform 3D virtual models created by
computer-aided design (CAD) into physical 3D objects in a layer-by-layer manner …

Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Magnify is a universal molecular anchoring strategy for expansion microscopy

A Klimas, BR Gallagher, P Wijesekara, S Fekir… - Nature …, 2023 - nature.com
Expansion microscopy enables nanoimaging with conventional microscopes by physically
and isotropically magnifying preserved biological specimens embedded in a crosslinked …

Integration of spatial and single-cell transcriptomic data elucidates mouse organogenesis

T Lohoff, S Ghazanfar, A Missarova, N Koulena… - Nature …, 2022 - nature.com
Molecular profiling of single cells has advanced our knowledge of the molecular basis of
development. However, current approaches mostly rely on dissociating cells from tissues …

Tissue clearing

DS Richardson, W Guan, K Matsumoto, C Pan… - Nature Reviews …, 2021 - nature.com
Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

PLD3 affects axonal spheroids and network defects in Alzheimer's disease

P Yuan, M Zhang, L Tong, TM Morse, RA McDougal… - Nature, 2022 - nature.com
The precise mechanisms that lead to cognitive decline in Alzheimer's disease are unknown.
Here we identify amyloid-plaque-associated axonal spheroids as prominent contributors to …

Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems

S Alon, DR Goodwin, A Sinha, AT Wassie, F Chen… - Science, 2021 - science.org
INTRODUCTION Cells and tissues are made up of diverse molecular building blocks,
organized with nanoscale precision over extended length scales. Newly developed …