Lysosomes are the main proteolytic compartments of mammalian cells comprising of a
battery of hydrolases. Lysosomes dispose and recycle extracellular or intracellular …

Mucopolysaccharidosis II, also known as Hunter syndrome, is a rare, X-linked disorder
caused by a deficiency of the lysosomal enzyme iduronate-2-sulfatase, which catalyzes a …

We describe a method for modifying proteins site-specifically using a chemoenzymatic
bioconjugation approach. Formylglycine generating enzyme (FGE) recognizes a …

Defects in lysosomal function have been associated with numerous monogenic human
diseases typically classified as lysosomal storage diseases. However, there is increasing …

Sulfatases cleave sulfate groups from various molecules and constitute a biologically and
industrially important group of enzymes. However, the number of sulfatases whose substrate …

Background The TRIM family is composed of multi-domain proteins that display the Tripartite
Motif (RING, B-box and Coiled-coil) that can be associated with a C-terminal domain. TRIM …

Formylglycine (fGly) is a catalytically essential residue found almost exclusively in the active
sites of type I sulfatases. Formed by post-translational oxidation of cysteine or serine side …

Importance of the field: Sulf-1 and Sulf-2 are sulfatases that edit the sulfation status of
heparan sulfate proteoglycans (HSPGs) on the outside of cells and regulate a number of …

Heparan sulfate (HS) is a cell surface carbohydrate polymer modified with sulfate moieties
whose highly ordered composition is central to directing specific cell signaling events. The …

Brassica plants have glucosinolate (GLs)-myrosinase defense mechanisms to deter
herbivores. However, Plutella xylostella specifically feeds on Brassica vegetables. The …