The classical genetic approach for exploring biological pathways typically begins by
identifying mutations that cause a phenotype of interest. Overexpression or misexpression of …

All successful cancer therapies are limited by the development of drug resistance. The
increase in the understanding of the molecular and biochemical bases of drug efficacy has …

While the catalog of mammalian transcripts and their expression levels in different cell types
and disease states is rapidly expanding, our understanding of transcript function lags …

Identifying the molecular targets for the beneficial or detrimental effects of small-molecule
drugs is an important and currently unmet challenge. We have developed a method, drug …

The attachment of glycans to asparagine residues of proteins is an abundant and highly
conserved essential modification in eukaryotes. The N-glycosylation process includes two …

The oligosaccharide substrate for the N-linked protein glycosylation is assembled at the
membrane of the endoplasmic reticulum. Dolichyl pyrophosphate serves as a carrier in this …

The yeast Saccharomyces cerevisiae is a powerful experimental system to study
biochemical, cell biological and molecular biological aspects of lipid synthesis. Most but not …

Peptidoglycan is a defining feature of the bacterial cell wall. Initially identified as a target of
the revolutionary beta-lactam antibiotics, peptidoglycan has become a subject of much …

Lowering the dosage of a single gene from two copies to one copy in diploid yeast results in
a heterozygote that is sensitized to any drug that acts on the product of this gene. This …

Selective protein kinase inhibitors were developed on the basis of the unexpected binding
mode of 2, 6, 9-trisubstituted purines to the adenosine triphosphate–binding site of the …