Due to its clear inherited backgrounds as well as simple and diverse genetic manipulation
systems, Bacillus subtilis is the key Gram-positive model bacterium for studies on physiology …

Competitive sustainable production in industry demands new and better biocatalysts,
optimized bioprocesses and cost-effective product recovery. Our review sheds light on the …

Here we describe a straightforward, efficient, and reliable way to clone an insert of choice
into a plasmid of choice without restriction endonucleases or T4 DNA ligase. Chimeric …

Gene replacement via homologous double crossover in filamentous fungi requires relatively
long (preferentially> 0.5 kb) flanking regions of the target gene. For this reason, gene …

The environmental threat posed by disposal of plastic wastes has drawn extensive attention
in recent years wherein polyethylene terephthalate (PET) constitutes one of the major plastic …

High-throughput genomics, proteomics and synthetic biology studies require ever more
efficient and economical strategies to clone complex DNA libraries or variants of biological …

The establishment of a clustered regularly interspaced short palindromic repeat (CRISPR)-
Cas9 system for strain construction in Bacillus subtilis is essential for its progression toward …

We present a method that allows simultaneous fusion and cloning of multiple PCR products
in a rapid and efficient manner. The procedure is based on the use of PCR primers that …

We have developed a fast and accurate method to engineer the Bacillus subtilis genome
that involves fusing by PCR two flanking homology regions with an antibiotic resistance …

We developed a general restriction enzyme-free and ligase-free method for subcloning up to
three DNA fragments into any location of a plasmid. The DNA multimer generated by …