The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of
recombinant proteins has become increasing popular in recent times. P. pastoris is easier to …

▪ Abstract An unconventional mechanism for retaining improperly folded glycoproteins and
facilitating acquisition of their native tertiary and quaternary structures operates in the …

Fed‐batch Chinese hamster ovary (CHO) cell culture is the most commonly used process for
IgG production in the biopharmaceutical industry. Amino acid and glucose consumption, cell …

The biophysical rules that govern folding of small, single‐domain proteins in dilute solutions
are now quite well understood. The mechanisms underlying co‐translational folding of …

The methylotrophic yeast Pichia pastoris is an established protein expression host for the
production of industrial enzymes. This yeast can be grown to very high cell densities and …

A common T17A polymorphism in the signal peptide of the cytotoxic T-lymphocyte antigen 4
(CTLA-4), a T-cell receptor that negatively regulates immune responses, is associated with …

In Saccharomyces cerevisiae, transfer of N-linked oligosaccharides is immediately followed
by trimming of ER-localized glycosidases. We analyzed the influence of specific …

▪ Abstract The endoplasmic reticulum (ER) serves as a way-station during the biogenesis of
nearly all secreted proteins, and associated with or housed within the ER are factors …

It has been proposed that cytoplasmic peptide: N-glycanase (PNGase) may be involved in
the proteasome-dependent quality control machinery used to degrade newly synthesized …

Protein and lipid glycosylation is a ubiquitous phenomenon. The task of cataloguing the
great structural variety of the glycan part has demanded considerable efforts over decades …