Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications

D Mark, S Haeberle, G Roth, F Von Stetten… - Microfluidics based …, 2010 - Springer
This review summarizes recent developments in microfluidic platform approaches. In
contrast to isolated application-specific solutions, a microfluidic platform provides a set of …

Single-cell barcoding and sequencing using droplet microfluidics

R Zilionis, J Nainys, A Veres, V Savova, D Zemmour… - Nature protocols, 2017 - nature.com
Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …

Single-cell analysis and sorting using droplet-based microfluidics

L Mazutis, J Gilbert, WL Ung, DA Weitz, AD Griffiths… - Nature protocols, 2013 - nature.com
We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

Droplet microfluidics—A tool for single‐cell analysis

HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …

Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology

AB Theberge, F Courtois, Y Schaerli… - Angewandte Chemie …, 2010 - Wiley Online Library
Microdroplets in microfluidics offer a great number of opportunities in chemical and
biological research. They provide a compartment in which species or reactions can be …

Upconverting nanoparticles for nanoscale thermometry

LH Fischer, GS Harms… - Angewandte Chemie …, 2011 - Wiley Online Library
Upconverting materials are capable of absorbing near‐infrared light and converting it into
short‐wavelength luminescence. The efficiency of this remarkable effect is highly …

Droplet microfluidics for microbiology: techniques, applications and challenges

TS Kaminski, O Scheler, P Garstecki - Lab on a Chip, 2016 - pubs.rsc.org
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

Two-phase microfluidic flows

CX Zhao, APJ Middelberg - Chemical Engineering Science, 2011 - Elsevier
Two phase systems are ubiquitous in processes and products, and in both cases
performance is maximized when precise control over the individual phases, and the …

Recent advances in function-based metagenomic screening

TR Ngara, H Zhang - Genomics, proteomics and bioinformatics, 2018 - academic.oup.com
Metagenomes from uncultured microorganisms are rich resources for novel enzyme genes.
The methods used to screen the metagenomic libraries fall into two categories, which are …