The CRISPR-Cas system is commonly known for its ability to cleave DNA in a
programmable manner, which has democratized gene editing and facilitated recent …

We set out to exhaustively characterize the impact of the cis-chromatin environment on
prime editing, a precise genome engineering tool. Using a highly sensitive method for …

Liquid–liquid phase separation (LLPS) plays a critical role in regulating gene transcription
via the formation of transcriptional condensates. However, LLPS has not been reported to be …

Recombinant adeno-associated virus (rAAV) vectors are gene therapy delivery tools that
offer a promising platform for the treatment of neurodegenerative diseases. Keeping up with …

New, orthogonal transcription factors in eukaryotic cells have been realized by engineering
nuclease-deficient CRISPR-associated proteins and/or their guide RNAs. In this work, we …

Cell-free gene expression systems are used in numerous applications, including medicine
making, diagnostics, and educational kits. Accurate quantification of nonfluorescent proteins …

CRISPR/Cas12a-based nucleic acid assays have been increasingly used for molecular
diagnostics. However, most current CRISPR/Cas12a-based RNA assays require the …

Engineering histone acylation states can inform mechanistic epigenetics and catalyze
therapeutic epigenome editing opportunities. Here, we developed engineered lysine …

Engineered transactivation domains (TADs) combined with programmable DNA binding
platforms have revolutionized synthetic transcriptional control. Despite recent progress in …

To assist in the establishment of an efficient CRISPR/Cas9 gene editing workflow in bovine
cells, we compared the efficiency of four Streptococcus pyogenes Cas9 (SpCas9) nuclease …