[HTML][HTML] Synthetic biology tools for novel secondary metabolite discovery in Streptomyces
Streptomyces are attractive microbial cell factories that have industrial capability to produce
a wide array of bioactive secondary metabolites. However, the genetic potential of the …
a wide array of bioactive secondary metabolites. However, the genetic potential of the …
Identifying small open reading frames in prokaryotes with ribosome profiling
Small proteins encoded by open reading frames (ORFs) shorter than 50 codons (small
ORFs [sORFs]) are often overlooked by annotation engines and are difficult to characterize …
ORFs [sORFs]) are often overlooked by annotation engines and are difficult to characterize …
Cryo-EM structure of the RADAR supramolecular anti-phage defense complex
RADAR is a two-protein bacterial defense system that was reported to defend against phage
by" editing" messenger RNA. Here, we determine cryo-EM structures of the RADAR defense …
by" editing" messenger RNA. Here, we determine cryo-EM structures of the RADAR defense …
Premature termination of transcription is shaped by Rho and translated uORFS in Mycobacterium tuberculosis
A D'Halluin, P Polgar, T Kipkorir, Z Patel, T Cortes… - Iscience, 2023 - cell.com
Little is known about the decisions behind transcription elongation versus termination in the
human pathogen Mycobacterium tuberculosis (M. TB). By applying Term-seq to M. TB we …
human pathogen Mycobacterium tuberculosis (M. TB). By applying Term-seq to M. TB we …
A rhlI 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in Pseudomonas aeruginosa
ABSTRACT N-Acyl homoserine lactone (AHL) quorum sensing (QS) controls expression of
over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the …
over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the …
The T-box riboswitch: tRNA as an effector to modulate gene regulation
KD Kreuzer, TM Henkin - Microbiology spectrum, 2018 - Am Soc Microbiol
The T-box riboswitch is a unique, RNA-based regulatory mechanism that modulates
expression of a wide variety of amino acid-related genes, predominantly in Firmicutes. RNAs …
expression of a wide variety of amino acid-related genes, predominantly in Firmicutes. RNAs …
Bacterial adaptation to antibiotics through regulatory RNAs
B Felden, V Cattoir - Antimicrobial agents and chemotherapy, 2018 - Am Soc Microbiol
The extensive use of antibiotics has resulted in a situation where multidrug-resistant
pathogens have become a severe menace to human health worldwide. A deeper …
pathogens have become a severe menace to human health worldwide. A deeper …
Type I toxin-antitoxin systems: regulating toxin expression via Shine-Dalgarno sequence sequestration and small RNA binding
S Masachis, F Darfeuille - Microbiology Spectrum, 2018 - Am Soc Microbiol
Toxin-antitoxin (TA) systems are small genetic loci composed of two adjacent genes: a toxin
and an antitoxin that prevents toxin action. Despite their wide distribution in bacterial …
and an antitoxin that prevents toxin action. Despite their wide distribution in bacterial …
Beyond self-resistance: ABCF ATPase LmrC is a signal-transducing component of an antibiotic-driven signaling cascade accelerating the onset of lincomycin …
M Koberska, L Vesela, V Vimberg, J Lenart, J Vesela… - MBio, 2021 - Am Soc Microbiol
In natural environments, antibiotics are important means of interspecies competition. At
subinhibitory concentrations, they act as cues or signals inducing antibiotic production; …
subinhibitory concentrations, they act as cues or signals inducing antibiotic production; …
Antibiotic stimulation of a Bacillus subtilis migratory response
Y Liu, S Kyle, PD Straight - MSphere, 2018 - Am Soc Microbiol
Competitive interactions between bacteria reveal physiological adaptations that benefit
fitness. Bacillus subtilis is a Gram-positive species with several adaptive mechanisms for …
fitness. Bacillus subtilis is a Gram-positive species with several adaptive mechanisms for …