Plasmids are important “vehicles” for the communication of genetic information between
bacteria. The exchange of plasmids transmits pathogenically and environmentally relevant …

An essential feature of bacterial plasmids is their ability to replicate as autonomous genetic
elements in a controlled way within the host. Therefore, they can be used to explore the …

Conjugative transfer of bacterial plasmids is the most efficient way of horizontal gene
spread, and it is therefore considered one of the major reasons for the increase in the …

The recent enormous expansion of the microbial DNA databases has made it profitable to
search for homologues and paralogues (homologues within species) of interesting genes. In …

High-and low-copy-number shuttle cloning vectors were constructed by incorporating the
Escherichia coli P15A plasmid origin of replication into the pAMβ1-derived vectors, pIL252 …

Publisher Summary The classical way of protein purification starting from a large number of
cells producing the protein using its authentic expression signals has been replaced by …

The identification, cloning, and characterization of protein toxins from various species of
bacilli have demonstrated the existence of mosquitocidal toxins with different structures …

The complete 50,237-bp DNA sequence of the conjugative and mobilizing multiresistance
plasmid pRE25 from Enterococcus faecalis RE25 was determined. The plasmid had 58 …

Transposon Tn917-LTV1 was used to produce a collection of Lactococcus lactis strains with
fusion of a promoterless lacZ gene to chromosomal loci. Screening 2,500 Tn917-LTV1 …

DNA replication in bacteria is carried out by a multiprotein complex, which is thought to
contain only one essential DNA polymerase, specified by the dnaE gene in Escherichia coli …