Isothermal titration calorimetry

M Bastos, O Abian, CM Johnson… - Nature Reviews …, 2023 - nature.com
Isothermal titration calorimetry (ITC) has become the gold standard for studying molecular
interactions in solution. Although it is increasingly being used in the soft matter and synthetic …

SEDPHAT–a platform for global ITC analysis and global multi-method analysis of molecular interactions

H Zhao, G Piszczek, P Schuck - Methods, 2015 - Elsevier
Isothermal titration calorimetry experiments can provide significantly more detailed
information about molecular interactions when combined in global analysis. For example …

High-precision isothermal titration calorimetry with automated peak-shape analysis

S Keller, C Vargas, H Zhao, G Piszczek… - Analytical …, 2012 - ACS Publications
Isothermal titration calorimetry (ITC) is a powerful classical method that enables researchers
in many fields to study the thermodynamics of molecular interactions. Primary ITC data …

Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions

CA Brautigam, H Zhao, C Vargas, S Keller, P Schuck - Nature protocols, 2016 - nature.com
Isothermal titration calorimetry (ITC) is a powerful and widely used method to measure the
energetics of macromolecular interactions by recording a thermogram of differential heating …

Binding affinity in drug design: experimental and computational techniques

V Kairys, L Baranauskiene… - Expert opinion on …, 2019 - Taylor & Francis
Introduction: In pharmaceutical design where future drugs are developed by targeting a
specific chosen protein, the evaluation of ligand affinity is crucial. For this very purpose are a …

Structure functional insights into calcium binding during the activation of coagulation factor XIII A

S Singh, J Dodt, P Volkers, E Hethershaw… - Scientific reports, 2019 - nature.com
The dimeric FXIII-A2, a pro-transglutaminase is the catalytic part of the heterotetrameric
coagulation FXIII-A2B2 complex that upon activation by calcium binding/thrombin cleavage …

Biomolecule–nanoparticle interactions: Elucidation of the thermodynamics by isothermal titration calorimetry

R Huang, BLT Lau - Biochimica et Biophysica Acta (BBA)-General Subjects, 2016 - Elsevier
Abstract Background Nanomaterials (NMs) are often exposed to a broad range of
biomolecules of different abundances. Biomolecule sorption driven by various interfacial …

[HTML][HTML] Pillar [6] MaxQ: a potent supramolecular host for in vivo sequestration of methamphetamine and fentanyl

AT Brockett, W Xue, D King, CL Deng, C Zhai… - Chem, 2023 - cell.com
Summary Pillar [6] MaxQ (P6AS) functions as an in vivo sequestration agent for
methamphetamine and fentanyl. We use 1 H NMR, isothermal titration calorimetry, and …

Pillar[n]MaxQ: A New High Affinity Host Family for Sequestration in Water

W Xue, PY Zavalij, L Isaacs - … Chemie International Edition, 2020 - Wiley Online Library
We report the synthesis, X‐ray crystal structure, and molecular recognition properties of
pillar [n] arene derivative P [6] AS, which we refer to as Pillar [6] MaxQ along with analogues …

Targeting and covalently immobilizing the EGFR through SNAP-tag technology for screening drug leads

J Fu, Q Jia, P Liang, S Wang, H Zhou, L Zhang… - Analytical …, 2021 - ACS Publications
Membrane protein immobilization is particularly significant in in vitro drug screening and
determining drug–receptor interactions. However, there are still some problems in the …