[HTML][HTML] Revealing bacterial cell biology using cryo-electron tomography

K Khanna, E Villa - Current Opinion in Structural Biology, 2022 - Elsevier
Visualizing macromolecules inside bacteria at a high spatial resolution has remained a
challenge owing to their small size and limited resolution of optical microscopy techniques …

[HTML][HTML] Capturing actin assemblies in cells using in situ cryo-electron tomography

J Schneider, M Jasnin - European Journal of Cell Biology, 2022 - Elsevier
Actin contributes to an exceptionally wide range of cellular processes through the assembly
and disassembly of highly dynamic and ordered structures. Visualizing these structures in …

Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies

M Toro-Nahuelpan, I Zagoriy, F Senger, L Blanchoin… - Nature …, 2020 - nature.com
Spatially controlled cell adhesion on electron microscopy supports remains a bottleneck in
specimen preparation for cellular cryo-electron tomography. Here, we describe contactless …

[HTML][HTML] Mechanistic insights into actin force generation during vesicle formation from cryo-electron tomography

D Serwas, M Akamatsu, A Moayed, K Vegesna… - Developmental cell, 2022 - cell.com
Actin assembly provides force for a multitude of cellular processes. Compared to actin-
assembly-based force production during cell migration, relatively little is understood about …

[HTML][HTML] Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET data

G Dimchev, B Amiri, F Fäßler, M Falcke… - Journal of Structural …, 2021 - Elsevier
A precise quantitative description of the ultrastructural characteristics underlying biological
mechanisms is often key to their understanding. This is particularly true for dynamic extra …

The architecture of traveling actin waves revealed by cryo-electron tomography

M Jasnin, F Beck, M Ecke, Y Fukuda… - Structure, 2019 - cell.com
Actin waves are dynamic supramolecular structures involved in cell migration, cytokinesis,
adhesion, and neurogenesis. Although wave-like propagation of actin networks is a …

Cryoelectron tomography reveals nanoscale organization of the cytoskeleton and its relation to microtubule curvature inside cells

S Chakraborty, J Mahamid, W Baumeister - Structure, 2020 - cell.com
Microtubules (MTs) are the most rigid elements of the cytoskeleton with in vitro persistence
lengths (L p) in the range of 1–6 mm. In cellular environments, however, MTs often appear …

Actin organization in cells responding to a perforated surface, revealed by live imaging and cryo-electron tomography

M Jasnin, M Ecke, W Baumeister, G Gerisch - Structure, 2016 - cell.com
In a 3D environment, motile cells accommodate their protruding and retracting activities to
geometrical cues. Dictyostelium cells migrating on a perforated film explored its holes by …

[HTML][HTML] Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: A review

B Özdemir, R Reski - Computational and structural biotechnology journal, 2021 - Elsevier
Cytoskeletal filaments are structures of utmost importance to biological cells and organisms
due to their versatility and the significant functions they perform. These biopolymers are most …

Three‐dimensional organization of the cytoskeleton: a cryo‐electron tomography perspective

S Chakraborty, M Jasnin, W Baumeister - Protein Science, 2020 - Wiley Online Library
Traditionally, structures of cytoskeletal components have been studied ex situ, that is, with
biochemically purified materials. There are compelling reasons to develop approaches to …