Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

L Li, S Wu, Y Si, H Li, X Yin… - Comprehensive Reviews in …, 2022 - Wiley Online Library
Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐
molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) …

Evolving a peptide: library platforms and diversification strategies

K Bozovičar, T Bratkovič - International journal of molecular sciences, 2019 - mdpi.com
Peptides are widely used in pharmaceutical industry as active pharmaceutical ingredients,
versatile tools in drug discovery, and for drug delivery. They find themselves at the …

Current technological improvements in enzymes toward their biotechnological applications

M Baweja, L Nain, Y Kawarabayasi… - Frontiers in …, 2016 - frontiersin.org
Enzymes from extremophiles are creating interest among researchers due to their unique
properties and the enormous power of catalysis at extreme conditions. Since community …

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method

S Naz, P Liu, U Farooq, H Ma - Microbial Cell Factories, 2023 - Springer
Regulation of amino acid's biosynthetic pathway is of significant importance to maintain
homeostasis and cell functions. Amino acids regulate their biosynthetic pathway by end …

Cognizance of molecular methods for the generation of mutagenic phage display antibody libraries for affinity maturation

CC Lim, YS Choong, TS Lim - International Journal of Molecular Sciences, 2019 - mdpi.com
Antibodies leverage on their unique architecture to bind with an array of antigens. The
strength of interaction has a direct relation to the affinity of the antibodies towards the …

Fitness selection of hyperfusogenic measles virus F proteins associated with neuropathogenic phenotypes

S Ikegame, T Hashiguchi, CT Hung… - Proceedings of the …, 2021 - National Acad Sciences
Measles virus (MeV) is resurgent and caused> 200,000 deaths in 2019. MeV infection can
establish a chronic latent infection of the brain that can recrudesce months to years after …

MAIVeSS: streamlined selection of antigenically matched, high-yield viruses for seasonal influenza vaccine production

C Gao, F Wen, M Guan, B Hatuwal, L Li… - Nature …, 2024 - nature.com
Vaccines are the main pharmaceutical intervention used against the global public health
threat posed by influenza viruses. Timely selection of optimal seed viruses with matched …

A Y161F hemagglutinin substitution increases thermostability and improves yields of 2009 H1N1 influenza A virus in cells

F Wen, L Li, N Zhao, MJ Chiang, H Xie… - Journal of …, 2018 - Am Soc Microbiol
Vaccination is the primary strategy for influenza prevention and control. However, egg-
based vaccines, the predominant production platform, have several disadvantages …

Forty years of directed evolution and its continuously evolving technology toolbox: A review of the patent landscape

Z Iqbal, S Sadaf - Biotechnology and Bioengineering, 2022 - Wiley Online Library
Generating functional protein variants with novel or improved characteristics has been a
goal of the biotechnology industry and life sciences, for decades. Rational design and …

Development of an Enhanced High-Yield Influenza Vaccine Backbone in Embryonated Chicken Eggs

L Guan, J Ping, TJS Lopes, S Fan, R Presler… - Vaccines, 2023 - mdpi.com
Vaccination is an efficient approach to preventing influenza virus infections. Recently, we
developed influenza A and B virus vaccine backbones that increased the yield of several …