Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …

Single-particle electron cryomicroscopy (cryo-EM) is an increasingly popular technique for
elucidating the three-dimensional (3D) structure of proteins and other biologically significant …

Photosystem II (PSII) is a light-driven protein, involved in the primary reactions of
photosynthesis. In plant photosynthetic membranes PSII forms large multisubunit …

Cryogenic electron tomography (cryo-ET) allows structural determination of biomolecules in
their native environment (in situ). Its potential of providing information on the dynamics of …

Highlights•Approaches to the structure determination and modelling of macromolecular
assemblies depend on the resolution of their cryo-EM map.•Insights into structure dynamics …

Single-particle analysis by electron microscopy is a well established technique for analyzing
the three-dimensional structures of biological macromolecules. Besides its ability to produce …

Xmipp is an open-source software package consisting of multiple programs for processing
data originating from electron microscopy and electron tomography, designed and managed …

Abstract Hybrid Electron Microscopy Normal Mode Analysis (HEMNMA) method was
introduced in 2014. HEMNMA computes normal modes of a reference model (an atomic …

The structural and dynamical characterization of biomolecules holds central importance in
the endeavor to understand the molecular mechanisms regulating living systems. However …

Highlights•Recent cryo-EM technology allows near-atomic structural resolution of many
complexes.•Conformational changes, functionally relevant, hinder higher resolution of …