Structural biology of CRISPR–Cas immunity and genome editing enzymes
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …
a wide range of genome editing and biotechnological applications. In this Review, we …
Gaussian accelerated molecular dynamics: Principles and applications
Gaussian accelerated molecular dynamics (GaMD) is a robust computational method for
simultaneous unconstrained enhanced sampling and free energy calculations of …
simultaneous unconstrained enhanced sampling and free energy calculations of …
Allostery in its many disguises: from theory to applications
Allosteric regulation plays an important role in many biological processes, such as signal
transduction, transcriptional regulation, and metabolism. Allostery is rooted in the …
transduction, transcriptional regulation, and metabolism. Allostery is rooted in the …
[HTML][HTML] Structural basis for Cas9 off-target activity
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
Rational designs of in vivo CRISPR-Cas delivery systems
The CRISPR-Cas system initiated a revolution in genome editing when it was, for the first
time, demonstrated success in the mammalian cells. Today, scientists are able to readily edit …
time, demonstrated success in the mammalian cells. Today, scientists are able to readily edit …
Eigenvector centrality for characterization of protein allosteric pathways
Determining the principal energy-transfer pathways responsible for allosteric communication
in biomolecules remains challenging, partially due to the intrinsic complexity of the systems …
in biomolecules remains challenging, partially due to the intrinsic complexity of the systems …
Catalytic Mechanism of Non-Target DNA Cleavage in CRISPR-Cas9 Revealed by Ab Initio Molecular Dynamics
CRISPR-Cas9 is a cutting-edge genome editing technology, which uses the endonuclease
Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is …
Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is …
CRISPR/Cas9 delivery system engineering for genome editing in therapeutic applications
H Cheng, F Zhang, Y Ding - Pharmaceutics, 2021 - mdpi.com
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein
9 (CRISPR/Cas9) systems have emerged as a robust and versatile genome editing platform …
9 (CRISPR/Cas9) systems have emerged as a robust and versatile genome editing platform …
Comparative perturbation-based modeling of the SARS-CoV-2 spike protein binding with host receptor and neutralizing antibodies: Structurally adaptable allosteric …
GM Verkhivker, S Agajanian, DY Oztas, G Gupta - Biochemistry, 2021 - ACS Publications
In this study, we used an integrative computational approach to examine molecular
mechanisms and determine functional signatures underlying the role of functional residues …
mechanisms and determine functional signatures underlying the role of functional residues …
AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation
W Sun, Z Cheng, J Wang, J Yang, X Li… - Proceedings of the …, 2023 - National Acad Sciences
Anti-CRISPR (Acr) proteins are encoded by phages and other mobile genetic elements and
inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum …
inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum …