In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …

Aminoacyl-tRNA synthetases (aaRS) ensure the faithful transmission of genetic information
in all living cells. The 24 known aaRS families are divided into 2 structurally distinct classes …

Proteins in living cells can be made receptive to bioorthogonal chemistries through
metabolic labeling with appropriately designed noncanonical amino acids (ncAAs). In the …

Aminoacyl-tRNA synthetases (aaRS) are the enzymes that ensure faithful transmission of
genetic information in all living cells, and are central to the developing technologies for …

Noncanonical amino acids (ncAAs) are synthetic building blocks that, when incorporated
into proteins, confer novel functions and enable precise control over biological processes …

Aminoacyl-tRNA synthetases (aaRSs) are modular enzymes globally conserved in the three
kingdoms of life. All catalyze the same two-step reaction, ie, the attachment of a …

The chemical and physical properties of proteins are limited by the 20 canonical amino
acids. Genetic code manipulation allows for the incorporation of noncanonical amino acids …

In this comprehensive review, I focus on the twenty E. coli aminoacyl‐tRNA synthetases and
their ability to charge non‐canonical amino acids (ncAAs) onto tRNAs. The promiscuity of …

Leishmania parasites cause two million new cases of leishmaniasis each year with several
hundreds of millions of people at risk. Due to the paucity and shortcomings of available …

Reagents and methods for the digital analysis of proteins or peptides are provided.
Specifically provided herein are proteins for identifying the N-terminal amino acid or N …