Digital droplet PCR in hematologic malignancies: a new useful molecular tool

S Galimberti, S Balducci, F Guerrini, M Del Re… - Diagnostics, 2022 - mdpi.com
Digital droplet PCR (ddPCR) is a recent version of quantitative PCR (QT-PCR), useful for
measuring gene expression, doing clonality assays and detecting hot spot mutations. In …

Twenty years of evolution of CML therapy: how the treatment goal is moving from disease to patient

D Russo, M Malagola, N Polverelli… - Therapeutic …, 2023 - journals.sagepub.com
The introduction of imatinib in 2000 opened the era of tyrosine kinase inhibitors (TKIs) for
CML therapy and has revolutionized the life expectancy of CML patients, which is now quite …

Minimal residual disease detection at RNA and leukemic stem cell (LSC) levels: comparison of RT-qPCR, d-PCR and CD26+ stem cell measurements in chronic …

E Abruzzese, M Bocchia, MM Trawinska, D Raspadori… - Cancers, 2023 - mdpi.com
Simple Summary The monitoring of the minimal residual disease (MRD) in Chronic Myeloid
Leukemia is a key element in the management of affected patients because it reflects the …

Effect of BCR::ABL1 transcript type and droplet digital polymerase chain reaction on successful treatment-free remission in chronic myeloid leukemia patients who …

H Park, HJ Kim, SK Sohn, Y Baik… - Therapeutic …, 2023 - journals.sagepub.com
Background: Droplet digital polymerase chain reaction (ddPCR) is an exact method of
measurement. Objectives: We conducted this study to identify the prognostic factors for …

SOHO State of the Art Updates and Next Questions: Chronic Myeloid Leukemia and Pregnancy:“Per Aspera Ad Astra”

E Abruzzese, MM Trawinska, P De Fabritiis… - … Myeloma and Leukemia, 2024 - Elsevier
Chronic myeloid leukemia (CML) has evolved from an invariably fatal disease to a chronic
disorder that can be treated with targeted drugs and allows survival expectations …

Artificial Intelligence-Based Management of Adult Chronic Myeloid Leukemia: Where Are We and Where Are We Going?

S Bernardi, M Vallati, R Gatta - Cancers, 2024 - mdpi.com
Simple Summary The field of artificial intelligence (AI) is quickly becoming recognized for its
potential to significantly improve medicine. AI is still in its infancy when it comes to treating …

Digital PCR as a new method for minimal residual disease monitoring and treatment free remission management in chronic myeloid leukemia patients: is it reliable?

S Bernardi, M Malagola, M Farina, N Polverelli, F Re… - Hemato, 2022 - mdpi.com
The effective and sensitive monitoring of Minimal Residual Disease or Measurable Residual
Disease (MRD) is a very important aspect in the management of patients affected by …

Investigation of co-flow step emulsification (CFSE) microfluidic device and its applications in digital polymerase chain reaction (ddPCR)

C Wei, W Lv, Y Ding, C Wang, C Sun, X Feng… - Journal of Colloid and …, 2025 - Elsevier
Hypothesis The co-flow step emulsification (CFSE) is very sensitive to the two-phase fluid
interfaces, we conjecture that the CFSE hydrodynamic model depends on several key …

Digital PCR (dPCR) is able to anticipate the achievement of stable deep molecular response in adult chronic myeloid leukemia patients: results of the …

S Bernardi, A Cavalleri, S Mutti, L Garuffo, M Farina… - Annals of …, 2024 - Springer
Abstract Chronic Myeloid Leukemia (CML) is marked by the BCR:: ABL1 fusion gene.
Monitoring tyrosine kinase inhibitor (TKI) therapy response is crucial for treatment …

Comparative analysis of BCR::ABL1 p210 mRNA transcript quantification and ratio to ABL1 control gene converted to the International Scale by chip digital PCR and …

W Saisaard, W Owattanapanich - Clinical Chemistry and Laboratory …, 2025 - degruyter.com
Objectives Chronic myeloid leukemia (CML) is characterized by the Philadelphia
chromosome, leading to the BCR:: ABL1 fusion gene and hyper-proliferation of …