Advances in nucleic acid amplification techniques (NAATs): COVID-19 point-of-care diagnostics as an example
Achieving superhigh sensitivity is the ultimate goal for bio-detection in modern analytical
science and life science. Among variable signal amplification strategies, nucleic acid …
science and life science. Among variable signal amplification strategies, nucleic acid …
CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
W Feng, AM Newbigging, J Tao, Y Cao, H Peng… - Chemical …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) protein systems have transformed the field of genome editing and …
associated (Cas) protein systems have transformed the field of genome editing and …
Mechanistic Insights into the cis-and trans-Acting DNase Activities of Cas12a
Summary CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …
Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing
YW Fu, XY Dai, WT Wang, ZX Yang… - Nucleic acids …, 2021 - academic.oup.com
Investigations of CRISPR gene knockout editing profiles have contributed to enhanced
precision of editing outcomes. However, for homology-directed repair (HDR) in particular …
precision of editing outcomes. However, for homology-directed repair (HDR) in particular …
Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair
Non-homologous end-joining (NHEJ) plays an important role in double-strand break (DSB)
repair of DNA. Recent studies have shown that the error patterns of NHEJ are strongly …
repair of DNA. Recent studies have shown that the error patterns of NHEJ are strongly …
CRISPR Cpf1 proteins: structure, function and implications for genome editing
F Safari, K Zare, M Negahdaripour… - Cell & bioscience, 2019 - Springer
CRISPR and CRISPR-associated (Cas) protein, as components of microbial adaptive
immune system, allows biologists to edit genomic DNA in a precise and specific way …
immune system, allows biologists to edit genomic DNA in a precise and specific way …
INDEL detection, the 'Achilles heel'of precise genome editing: a survey of methods for accurate profiling of gene editing induced indels
EP Bennett, BL Petersen, IE Johansen… - Nucleic acids …, 2020 - academic.oup.com
Advances in genome editing technologies have enabled manipulation of genomes at the
single base level. These technologies are based on programmable nucleases (PNs) that …
single base level. These technologies are based on programmable nucleases (PNs) that …
Catalytic Mechanism of Non-Target DNA Cleavage in CRISPR-Cas9 Revealed by Ab Initio Molecular Dynamics
CRISPR-Cas9 is a cutting-edge genome editing technology, which uses the endonuclease
Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is …
Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is …
Massively parallel kinetic profiling of natural and engineered CRISPR nucleases
Engineered SpCas9s and AsCas12a cleave fewer off-target genomic sites than natural
SpCas9. However, understanding their fidelity, mechanisms and cleavage outcomes …
SpCas9. However, understanding their fidelity, mechanisms and cleavage outcomes …
Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering
In the last years, tremendous progress has been made in the development of CRISPR/Cas-
mediated genome editing tools. A number of natural CRISPR/Cas nuclease variants have …
mediated genome editing tools. A number of natural CRISPR/Cas nuclease variants have …