Recent advances in development of marker-free transgenic plants: regulation and biosafety concern

N Tuteja, S Verma, RK Sahoo, S Raveendar… - Journal of …, 2012 - Springer
During the efficient genetic transformation of plants with the gene of interest, some
selectable marker genes are also used in order to identify the transgenic plant cells or …

Current achievements and future prospects of genetic engineering in Indian mustard (Brassica juncea L. Czern & Coss.)

AK Thakur, N Parmar, KH Singh, J Nanjundan - Planta, 2020 - Springer
Main conclusion Transgenic technology in Indian mustard has expedited crop improvement
programs. Further, there is a need to optimize gene editing protocols and find out the …

BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo candida

H Arora, KL Padmaja, K Paritosh, N Mukhi… - Theoretical and applied …, 2019 - Springer
Key message BjuWRR1, a CNL-type R gene, was identified from an east European gene
pool line of Brassica juncea and validated for conferring resistance to white rust by genetic …

Targeted silencing of BjMYB28 transcription factor gene directs development of low glucosinolate lines in oilseed Brassica juncea

R Augustine, A Mukhopadhyay… - Plant Biotechnology …, 2013 - Wiley Online Library
B rassica juncea (Indian mustard), a globally important oilseed crop, contains relatively high
amount of seed glucosinolates ranging from 80 to 120 μmol/g dry weight (DW). One of the …

Site-specific excisional recombination strategies for elimination of undesirable transgenes from crop plants

D Gidoni, V Srivastava, N Carmi - In Vitro Cellular & Developmental …, 2008 - Springer
A major limitation of crop biotechnology and breeding is the lack of efficient molecular
technologies for precise engineering of target genomic loci. While transformation …

An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.)

M Bhatnagar, K Prasad, P Bhatnagar-Mathur… - Plant cell reports, 2010 - Springer
Recombinant genes conferring resistance to antibiotics or herbicides are widely used as
selectable markers in plant transformation for selecting the primary transgenic events …

Suitability of non-lethal marker and marker-free systems for development of transgenic crop plants: present status and future prospects

P Manimaran, G Ramkumar, K Sakthivel… - Biotechnology …, 2011 - Elsevier
Genetically modified crops are one of the prudent options for enhancing the production and
productivity of crop plants by safeguarding from the losses due to biotic and abiotic stresses …

Generation of selectable marker-free sheath blight resistant transgenic rice plants by efficient co-transformation of a cointegrate vector T-DNA and a binary vector T …

R Sripriya, V Raghupathy, K Veluthambi - Plant cell reports, 2008 - Springer
Co-transformation of Oryza sativa L. var. Pusa Basmati1 was done using an Agrobacterium
tumefaciens strain harbouring a single-copy cointegrate vector and a multi-copy binary …

Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics

P Rawat, AK Singh, K Ray, B Chaudhary, S Kumar… - Journal of …, 2011 - Springer
High levels of expression of the cry1Ac gene from Bacillus thuringiensis cannot be routinely
achieved in transgenic plants despite modifications made in the gene to improve its …

DNA methylation occurring in Cre‐expressing cells inhibits loxP recombination and silences loxP‐sandwiched genes

R Liu, Q Long, X Zou, Y Wang, Y Pei - New Phytologist, 2021 - Wiley Online Library
The low DNA recombination efficiency of site‐specific recombinase systems in plants limits
their application; however, the underlying mechanism is unknown. We evaluate the gene …