Cellular function in tissue is dependent on the local environment, requiring new methods for
spatial mapping of biomolecules and cells in the tissue context. The emergence of spatial …

Although single-cell and spatial sequencing methods enable simultaneous measurement of
more than one biological modality, no technology can capture all modalities within the same …

Palatine tonsils are secondary lymphoid organs (SLOs) representing the first line of
immunological defense against inhaled or ingested pathogens. We generated an atlas of …

The development and deployment of single-cell genomic technologies have driven a
resolution revolution in our understanding of the immune system, providing unprecedented …

Studying the human immune system is challenging. These challenges stem from the
complexity of the immune system itself, the heterogeneity of the immune system between …

Fibroblastic reticular cells (FRCs) direct the interaction and activation of immune cells in
discrete microenvironments of lymphoid organs. Despite their important role in steering …

Protective immunity following vaccination is sustained by long-lived antibody-secreting cells
and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 …

Class-switch recombination (CSR) is an integral part of B cell maturation. Here we present
sciCSR (pronounced 'scissor', single-cell inference of class-switch recombination), a …

Lymphocytes can heavily influence intestinal health, but resolving intestinal lymphocyte
function is challenging as the intestine contains a vastly heterogeneous mixture of cells. Pigs …

B lymphocytes are essential mediators of humoral immunity and play multiple roles in
human cancer. To decode the functions of tumor-infiltrating B cells, we generated a B cell …