Highlights•Phototransformable fluorescent proteins undergo different light-induced
reactions.•These markers are largely used in super-resolution microscopy and …

Optical fluorescence microscopy has taken center stage in the exploration of biological
structure and dynamics, especially on live specimens, and super-resolution imaging …

Superresolution imaging methods—now widely used to characterize biological structures
below the diffraction limit—are poised to reveal in quantitative detail the stoichiometry of …

Advanced imaging techniques crucially depend on the labels used. In this work, we present
the structure-guided design of a fluorescent protein that displays both reversibly …

Green‐to‐red photoconvertible fluorescent proteins (FPs) are vital biomimetic tools for
powerful techniques such as super‐resolution imaging. A unique Kaede‐type FP named the …

Photoactivated localization microscopy (PALM) is a powerful technique to investigate
cellular nanostructures quantitatively and dynamically. However, the use of PALM for …

Red fluorescent proteins (RFPs) represent an increasingly popular class of genetically
encodable bioprobes and biomarkers that can advance next‐generation breakthroughs …

Photochromic fluorescent proteins (FPs) have proved to be indispensable luminous probes
for sophisticated and advanced bioimaging techniques. Among them, an interplay between …

The fluorescence microscopy methods presently used to characterize protein motion in cells
infer protein motion from indirect observables, rather than measuring protein motion directly …

Target identification and mechanistic studies of cytotoxic agents are challenging processes
that are both time-consuming and costly. Here we describe an approach to mechanism of …