Although the first ancient DNA molecules were extracted more than three decades ago, the
first ancient nuclear genomes could only be characterized after high-throughput sequencing …

Many species have undergone dramatic population size declines over the past centuries.
Although stochastic genetic processes during and after such declines are thought to elevate …

The grey wolf (Canis lupus) was the first species to give rise to a domestic population, and
they remained widespread throughout the last Ice Age when many other large mammal …

INTRODUCTION Genetic studies of the Pleistocene peopling of the Americas have focused
on the timing and number of migrations from Siberia into North America. They show that …

We present a protocol to prepare extracted DNA for sequencing on the Illumina sequencing
platform that has been optimized for ancient and degraded DNA. Our approach, the Santa …

Only five species of the once-diverse Rhinocerotidae remain, making the reconstruction of
their evolutionary history a challenge to biologists since Darwin. We sequenced genomes …

Here, we describe single-tube long fragment read (stLFR), a technology that enables
sequencing of data from long DNA molecules using economical second-generation …

Background More extensive use of metagenomic shotgun sequencing in microbiome
research relies on the development of high-throughput, cost-effective sequencing. Here we …

In the past decade, several studies have estimated the human per-generation germline
mutation rate using large pedigrees. More recently, estimates for various nonhuman species …

Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we
present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq …