The actin cytoskeleton provides the structural basis for cell polarity in Saccharomyces
cerevisiae as well as most other eukaryotes. In Part I of this two-part commentary, presented …

It is now well appreciated that derivatives of phosphatidylinositol (PtdIns) are key regulators
of many cellular processes in eukaryotes. Of particular interest are phosphoinositides (mono …

Three cell‐permeant compounds, cytochalasin D, latrunculin A and jasplakinolide, which
perturb intracellular actin dynamics by distinct mechanisms, were used to probe the role of …

Many endocytosed proteins in yeast travel to the vacuole, but some are recycled to the
plasma membrane. We have investigated the recycling of chimeras containing green …

Intracellular Ca2+ is normally maintained at submicromolar levels but increases during
many forms of cellular stimulation. This increased Ca2+ binds to receptor proteins such as …

The basic reaction mechanisms for membrane fusion in the trafficking of intracellular
membranes and in exocytosis are probably identical. But in contrast to regulated exocytosis …

Delivery of proteins to the vacuole of the yeast Saccharomyces cerevisiae provides an
excellent model system in which to study vacuole and lysosome biogenesis and membrane …

Emp24p is a type I transmembrane protein that is involved in secretory protein transport from
the endoplasmic reticulum (ER) to the Golgi complex. A yeast mutant that lacks Emp24p …

Previous studies have shown that temperature-sensitive, myo2-66 yeast arrest as large,
unbudded cells that accumulate vesicles within their cytoplasm (Johnston, GC, JA …

Type I myosins are thought to drive actin-dependent membrane motility, but the direct
demonstration in vivo of their involvement in specific cellular processes has been difficult …