Overcoming the pitfalls of merging dietary metabarcoding into ecological networks

JP Cuff, FM Windsor, MPTG Tercel… - Methods in Ecology …, 2022 - Wiley Online Library
The construction of increasingly detailed species interaction networks is extending the
potential applications of network ecology, providing an opportunity to understand complex …

Methodological overview and data‐merging approaches in the study of plant–frugivore interactions

E Quintero, J Isla, P Jordano - Oikos, 2022 - Wiley Online Library
Recording species interactions is one of the main challenges in ecological studies.
Frugivory has received much attention for decades as a model for mutualisms among free …

An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data

LE Drake, JP Cuff, RE Young… - Methods in Ecology …, 2022 - Wiley Online Library
Metabarcoding provides a powerful tool for investigating biodiversity and trophic
interactions, but the high sensitivity of this methodology makes it vulnerable to errors …

Current methods and future directions in avian diet analysis

BD Hoenig, AM Snider, AM Forsman, KA Hobson… - The Auk, 2022 - academic.oup.com
Identifying the composition of avian diets is a critical step in characterizing the roles of birds
within ecosystems. However, because birds are a diverse taxonomic group with equally …

Specific gut bacterial responses to natural diets of tropical birds

KH Bodawatta, I Klečková, J Klečka, K Pužejová… - Scientific reports, 2022 - nature.com
The composition of gut bacterial communities is strongly influenced by the host diet in many
animal taxa. For birds, the effect of diet on the microbiomes has been documented through …

Successes and limitations of quantitative diet metabarcoding in a small, herbivorous mammal

TE Stapleton, SB Weinstein… - Molecular Ecology …, 2022 - Wiley Online Library
DNA metabarcoding is widely used to determine wild animal diets, but whether this
technique provides accurate, quantitative measurements is still under debate. To test our …

The predator problem and PCR primers in molecular dietary analysis: swamped or silenced; depth or breadth?

JP Cuff, JJN Kitson… - Molecular Ecology …, 2023 - Wiley Online Library
Dietary metabarcoding has vastly improved our ability to analyse the diets of animals, but it
is hampered by a plethora of technical limitations including potentially reduced data output …

Handling of targeted amplicon sequencing data focusing on index hopping and demultiplexing using a nested metabarcoding approach in ecology

Y Guenay-Greunke, DA Bohan, M Traugott… - Scientific reports, 2021 - nature.com
High-throughput sequencing platforms are increasingly being used for targeted amplicon
sequencing because they enable cost-effective sequencing of large sample sets. For …

DNA metabarcoding reveals the importance of gelatinous zooplankton in the diet of Pandalus borealis, a keystone species in the Arctic

P Urban, K Præbel, S Bhat, J Dierking… - Molecular …, 2022 - Wiley Online Library
Abstract Information about the dietary composition of a species is crucial to understanding
their position and role in the food web. Increasingly, molecular approaches such as DNA …

DNA metabarcoding illuminates the contribution of small and very small prey taxa to the diet of lions

K Groen, S Beekenkamp, HH de Iongh… - Environmental …, 2023 - Wiley Online Library
Abstract Knowledge of food web interactions is essential for understanding the role of
carnivores in an ecosystem and designing appropriate conservation and management …