A number of common features are associated with a great diversity of observations of
somatic embryogenesis in vitro. There are fundamental homologies between direct and …

After a short introduction, the cytogenetics of plant cell and tissue cultures and their
regenerates will be discussed. In the first section discussion will focus on cytogenetic …

In the summer of 1983, immature embryos from 101 selfed inbred lines and germplasm
stocks of Zea mays L. were examined for their ability to produce callus cultures capable of …

Friable, embryogenic maize (Zea mays L.), inbred line A188, callus was established and
maintained for more than one year without apparent loss of friability or embryogenic …

Basal leaf segments of 3 to 4 week old maize (Zea mays L.) seedlings plated on SH medium
with 30 μM dicamba produced embryogenic callus and/or somatic embryos. Histological …

Growth and morphogenesis of plant tissue cultures can be improved by small amounts of
some organic nutrients. These are mainly vitamins (including some substances that are not …

In this paper, we describe the transformation of regenerable maize tissues by
electroporation. In many maize lines, immature zygotic embryos can give rise to …

Recent advances in cell culture and molecular biology of higher plants, which are key
components of plant biotechnology, have demonstrated the considerable power and …

We demonstrate here the possibility of regenerating phenotypically normal, fertile maize
plants via in vitro fertilization of isolated, single sperm and egg cells mediated by …

An efficient maize regeneration system was developed using mature embryos. Embryos
were removed from surface-sterilized mature seeds and sliced into halves. They were used …