The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
JF Huggett, CA Foy, V Benes, K Emslie… - Clinical …, 2013 - academic.oup.com
There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …
practical and increasingly affordable technology. dPCR allows the precise quantification of …
High throughput gene expression measurement with real time PCR in a microfluidic dynamic array
SL Spurgeon, RC Jones, R Ramakrishnan - PloS one, 2008 - journals.plos.org
We describe a high throughput gene expression platform based on microfluidic dynamic
arrays. This system allows 2,304 simultaneous real time PCR gene expression …
arrays. This system allows 2,304 simultaneous real time PCR gene expression …
RT-qPCR and RT-digital PCR: a comparison of different platforms for the evaluation of residual disease in chronic myeloid leukemia
BACKGROUND Tyrosine kinase inhibitors (TKIs) are the cornerstone of successful clinical
management of patients with chronic myeloid leukemia (CML). Quantitative monitoring of the …
management of patients with chronic myeloid leukemia (CML). Quantitative monitoring of the …
[HTML][HTML] Designing and interpretation of digital assays: Concentration of target in the sample and in the source of sample
PR Debski, P Garstecki - Biomolecular detection and quantification, 2016 - Elsevier
We explain how to design classic digital assays, comprising identical partitions, in order to
obtain the required precision of the estimate within a defined range of concentrations. The …
obtain the required precision of the estimate within a defined range of concentrations. The …
Rational design of digital assays
PR Debski, K Gewartowski, M Sulima… - Analytical …, 2015 - ACS Publications
Optimum algorithm for digital assays treats chemical compartments as bits of probabilistic
information and arranges these bits in a fractional positional system. Maximization of …
information and arranges these bits in a fractional positional system. Maximization of …
Direct elicitation of template concentration from quantification cycle (Cq) distributions in digital PCR
M Mojtahedi, A Fouquier d'Hérouël… - Nucleic Acids …, 2014 - academic.oup.com
Digital PCR (dPCR) exploits limiting dilution of a template into an array of PCR reactions.
From this array the number of reactions that contain at least one (as opposed to zero) initial …
From this array the number of reactions that contain at least one (as opposed to zero) initial …
[PDF][PDF] Biomolecular Detection and Quantification
PR Debski, P Garstecki - 2016 - cyberleninka.org
abstract We explain how to design classic digital assays, comprising identical partitions, in
order to obtain the required precision of the estimate within a defined range of …
order to obtain the required precision of the estimate within a defined range of …