ABSTRACT Y-box binding proteins are DNA-and RNA-binding proteins with an
evolutionarily ancient and conserved cold shock domain. The Y-box binding protein 1 (YB-1) …

Directed stabilization of globular proteins via substitution of a minimal number of amino acid
residues is one of the most complicated experimental tasks. In this work, we have …

Ice-binding proteins are crucial for the adaptation of various organisms to low temperatures.
Some of these, called antifreeze proteins, are usually thought to inhibit growth and/or …

The adaptation of organisms to sub-zero temperatures is an intriguing problem in biology
and biotechnology. The ice-binding antifreeze proteins are known to be responsible for the …

Ice-binding proteins are expressed in the cells of some cold adapted organisms, helping
them to survive at extremely low temperatures. One of the problems in studying such …

Antifreeze proteins, expressed in cold-blooded organisms, prevent ice formation in their
bodies, and thus help them to survive in extremely cold winter temperatures. However, the …

Ice-binding proteins are expressed in the cells of some organisms, helping them to survive
extremely low temperatures. One of the problems in study of such proteins is the difficulty of …

Multicopy episomal plasmids in yeast, used whenever elevated levels of foreign or
homologous gene expression are necessary, are known to be less stable compared to the …

Materials and methods Optimization of the hybrid protein was carried out by molecular
dynamics (MD) method in Gromacs 4.5. 3 program (Abraham et al., Citation 2015) using the …

Disclosed herein are methods and compositions comprising chimeric green fluorescent
protein (GFP) molecules embedded with foreign molecules. Also disclosed are methods of …