Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination
technique. Its most prolific branch is single particle analysis (SPA), a method being used in a …

This review summarizes the current state of methods and results achievable by cryo-electron
microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to …

Imaging large fields of view while preserving high-resolution structural information remains a
challenge in low-dose cryo-electron tomography. Here we present robust tools for montage …

INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Chloroplast function is orchestrated by the organelle's intricate architecture. By combining
cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron …

Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

Traditionally, macromolecular structure determination is performed ex situ, that is, with
purified materials. But, there are strong incentives to develop approaches to study them in …

Researchers have used transmission electron microscopy (TEM) to make contributions to
cell biology for well over 50 years, and TEM continues to be an important technology in our …