The SpyCatcher-SpyTag system was developed seven years ago as a method for protein
ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein …

Autotransport in Gram-negative bacteria denotes the ability of surface-localized proteins to
cross the outer membrane (OM) autonomously. Autotransporters perform this task with the …

Artificial multienzyme scaffolds are being developed for in vitro cascaded biocatalytic activity
and, in particular, accessing substrate channeling. This review covers progress in this field …

The highly acclaimed prospect of renewable lignocellulosic biocommodities as obvious
replacement of their fossil-based counterparts is burgeoning within the last few years …

Bacterial surface display entails the presentation of recombinant proteins or peptides on the
surface of bacterial cells. Escherichia coli is the most frequently used bacterial host for …

Controlled human malaria infection (CHMI) entails deliberate infection with malaria
parasites either by mosquito bite or by direct injection of sporozoites or parasitized …

Gram-negative bacteria are attractive hosts for recombinant protein production because they
are fast growing, easy to manipulate, and genetically stable in large cultures. However, the …

Mixed-acid fermentation end products have numerous applications in biotechnology. This is
probably the main driving force for the development of multiple strains that are supposed to …

Despite the first report on the bacterial display of a recombinant peptide appeared almost 30
years ago, industrial application of cells with surface-displayed enzymes is still limited. To …

The two membranes of Gram-negative bacteria contain protein machines that have a
general function in their assembly. To interact with the extra-cellular milieu, Gram-negatives …