Here we review recent advances in understanding the regulation of mRNA synthesis in
Saccharomyces cerevisiae. Many fundamental gene regulatory mechanisms have been …

The yeast genetics community has embraced genomic biology, and there is a general
understanding that obtaining a full encyclopedia of functions of the∼ 6000 genes is a …

Deep transcriptome sequencing has revealed the existence of many transcripts that lack
long or conserved open reading frames (ORFs) and which have been termed long non …

The identification of untranslated regions, introns, and coding regions within an organism
remains challenging. We developed a quantitative sequencing-based method called RNA …

Nucleosome organization is critical for gene regulation. In living cells this organization is
determined by multiple factors, including the action of chromatin remodellers, competition …

The nonrandom distribution of meiotic recombination influences patterns of inheritance and
genome evolution, but chromosomal features governing this distribution are poorly …

Eukaryotes transcribe much of their genomes, but little is known about the fidelity of
transcriptional initiation by RNA polymerase II in vivo. I suggest that∼ 90% of Pol II initiation …

The detailed positions of nucleosomes profoundly impact gene regulation and are partly
encoded by the genomic DNA sequence. However, less is known about the functional …

Chromatin allows the eukaryotic cell to package its DNA efficiently. To understand how
chromatin structure is controlled across the Saccharomyces cerevisiae genome, we have …

Previous studies in Saccharomyces cerevisiae have demonstrated that cryptic promoters
within coding regions activate transcription in particular mutants. We have performed a …