Circular Permutation of Bacillus circulans Xylanase: A Kinetic and Structural Study

S Reitinger, Y Yu, J Wicki, M Ludwiczek, I D'Angelo… - Biochemistry, 2010 - ACS Publications
The 20 kDa Bacillus circulans Bcx is a well-studied endoxylanase with a β-jellyroll fold that
places its N-and C-termini in salt bridge contact. Initial experiments verified that Bcx could be …

Backbone cyclization of Salmonella typhimurium diaminopropionate ammonia-lyase to enhance the activity and stability

X He, T Lin, Y Xie, J Li, Y Ge, S Zhang, J Fan - Protein expression and …, 2024 - Elsevier
Diaminopropionate ammonia-lyase transforms D and L isomers of 2, 3-diaminopropionate to
pyruvate and ammonia. It catalyzes D-and l-serine less effectively. L-2, 3-diaminopropionate …

Off‐pathway‐sensitive protein‐splicing screening based on a toxin/antitoxin system

HM Beyer, H Iwaï - ChemBioChem, 2019 - Wiley Online Library
Protein‐splicing domains are frequently used engineering tools that find application in the in
vivo and in vitro ligation of protein domains. Directed evolution is among the most promising …

The pivotal role of multimodality reporter sensors in drug discovery: from cell based assays to real time molecular imaging

P Ray - Current pharmaceutical biotechnology, 2011 - ingentaconnect.com
Development and marketing of new drugs require stringent validation that are expensive
and time consuming. Non-invasive multimodality molecular imaging using reporter genes …

16 Probing protein dynamics in vivo using backbone cyclization: bacterial acyl carrier protein as a case study

G Volkmann, PW Murphy, EE Rowland… - Methods in Protein …, 2011 - degruyter.com
Proteins are inherently flexible and structurally dynamic biomolecules. The ability to switch
between different conformational state s is often a pivotal feature for such diverse cellular …